ATP2C1 Protein (AA 313-699) (His tag)

Catalog No. ABIN3089348

Product Details

Target: ATP2C1 (ATPase, Ca++ Transporting, Type 2C, Member 1 (ATP2C1))

Protein Type: Recombinant

Protein Characteristics: AA 313-699

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ATP2C1 protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: MVVTVTLALG VMRMVKKRAI VKKLPIVETL GCCNVICSDK TGTLTKNEMT VTHIFTSDGL H AEVTGVGYNQ FGEVIVDGDV VHGFYNPAVS RIVEAGCVCN DAVIRNNTLM GKPTEGALIA LAMKMGLDGL QQDYIRKAEY PFSSEQKWMA VKCVHRTQQD RPEICFMKGA YEQVIKYCTT YQSKGQTLTL TQQQRDVYQQ EKARMGSAGL RVLALASGPE LGQLTFLGLV GIIDPPRTGV KEAVTTLIAS GVSIKMITGD SQETAVAIAS RLGLYSKTSQ SVSGEEIDAM DVQQLSQIVP KVAVFYRASP RHKMKIIKSL QKNGSVVAMT GDGVN
Sequence includes C-terminal His-tag

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PLP1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: The protein is purified from the cleared cell lysate using His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In solution (30 mM Hepes, pH 8.0, 100 mM NaCl, Laurylsarcosine)

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

References

Chernyavsky, Amber, Agnoletti, Wang, Grando: "Synergy among non-desmoglein antibodies contributes to the immunopathology of desmoglein antibody-negative pemphigus vulgaris." in: The Journal of biological chemistry, Vol. 294, Issue 12, pp. 4520-4528, (2019) (PubMed).

Target Details

Target: ATP2C1 (ATPase, Ca++ Transporting, Type 2C, Member 1 (ATP2C1))

Alternative Name: ATP2C1 (ATP2C1 Products)

Synonyms: ATP2C1 Protein, ATP2C1A Protein, BCPM Protein, HHD Protein, PMR1 Protein, SPCA1 Protein, hSPCA1 Protein, Spca1 Protein, si:dkey-11p23.6 Protein, SPCA Protein, 1700121J11Rik Protein, AW061228 Protein, D930003G21Rik Protein, pmr1 Protein, ATPase secretory pathway Ca2+ transporting 1 Protein, ATPase, Ca++ transporting, type 2C, member 1 Protein, ATPase, Ca++-sequestering Protein, ATP2C1 Protein, atp2c1 Protein, Atp2c1 Protein

Background: This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of the calcium.

Molecular Weight: 42.7 kDa including tag.

UniProt: P98194

Pathways: Transition Metal Ion Homeostasis, Ribonucleoside Biosynthetic Process