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Asialoglycoprotein Receptor 2 Protein (ASGR2) (AA 80-311) (His tag)

Crystallography grade ASGR2 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3089518
  • Target See all Asialoglycoprotein Receptor 2 (ASGR2) Proteins
    Asialoglycoprotein Receptor 2 (ASGR2)
    Protein Type
    Recombinant
    Protein Characteristics
    AA 80-311
    Origin
    • 6
    • 3
    • 1
    Human
    Source
    • 3
    • 3
    • 2
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This Asialoglycoprotein Receptor 2 protein is labelled with His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    QSEGHGGAQL QAELRSLKEA FSNFSSSTLT EVQAISTHGG SVGDKITSLG AKLEKQQQDL KADHDALLFH LKHFPVDLRF VACQMELLHS NGSQRTCCPV NWVEHQGSCY WFSHSGKAWA EAEKYCQLEN AHLVVINSWE EQKFIVQHTN PFNTWIGLTD SDGSWKWVDG TDYRHNYKNW AVTQPDNWHG HELGGSEDCV EVQPDGRWND DFCLQVYRWV CEKRRNATGE VA
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human ASGR2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product ASGR2 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    Asialoglycoprotein Receptor 2 (ASGR2)
    Alternative Name
    ASGR2 (ASGR2 Products)
    Synonyms
    ASGR2 Protein, asgr2 Protein, MGC137129 Protein, ASGP-R2 Protein, ASGPR2 Protein, CLEC4H2 Protein, HBXBP Protein, HL-2 Protein, Asgr Protein, Asgr-2 Protein, asialoglycoprotein receptor 2 Protein, C-type lectin domain family 10 member A Protein, ASGR2 Protein, clec10a Protein, Asgr2 Protein, LOC100347178 Protein
    Background
    Mediates the endocytosis of plasma glycoproteins to which the terminal sialic acid residue on their complex carbohydrate moieties has been removed. The receptor recognizes terminal galactose and N-acetylgalactosamine units. After ligand binding to the receptor, the resulting complex is internalized and transported to a sorting organelle, where receptor and ligand are disassociated. The receptor then returns to the cell membrane surface.
    Molecular Weight
    27.4 kDa Including tag.
    UniProt
    P07307
    Pathways
    Thyroid Hormone Synthesis
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