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BMPR1A Protein (AA 177-532) (His tag)

Crystallography grade BMPR1A Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Catalog No. ABIN3089857
  • Target See all BMPR1A Proteins
    BMPR1A (Bone Morphogenetic Protein Receptor 1A (BMPR1A))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 177-532
    Origin
    • 23
    • 8
    • 2
    • 1
    • 1
    Human
    Source
    • 10
    • 6
    • 6
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This BMPR1A protein is labelled with His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Sequence
    KHYCKSISSR RRYNRDLEQD EAFIPVGESL KDLIDQSQSS GSGSGLPLLV QRTIAKQIQM VRQVGKGRYG EVWMGKWRGE KVAVKVFFTT EEASWFRETE IYQTVLMRHE NILGFIAADI KGTGSWTQLY LITDYHENGS LYDFLKCATL DTRALLKLAY SAACGLCHLH TEIYGTQGKP AIAHRDLKSK NILIKKNGSC CIADLGLAVK FNSDTNEVDV PLNTRVGTKR YMAPEVLDES LNKNHFQPYI MADIYSFGLI IWEMARRCIT GGIVEEYQLP YYNMVPSDPS YEDMREVVCV KRLRPIVSNR WNSDECLRAV LKLMSECWAH NPASRLTALR IKKTLAKMVE SQDVKI
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human BMPR1A Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product BMPR1A Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    BMPR1A (Bone Morphogenetic Protein Receptor 1A (BMPR1A))
    Alternative Name
    BMPR1A (BMPR1A Products)
    Synonyms
    10q23del Protein, ACVRLK3 Protein, ALK3 Protein, CD292 Protein, SKR5 Protein, 1110037I22Rik Protein, AU045487 Protein, BMPR-IA Protein, Bmpr Protein, BMPR-1 Protein, BRK-1 Protein, BMPR1A Protein, acvrlk3 Protein, alk-3 Protein, alk3 Protein, cd292 Protein, skr5 Protein, BR1a Protein, alk3tr Protein, bmpr1a Protein, zgc:136731 Protein, wu:fe05b06 Protein, wu:fe06e10 Protein, zgc:92227 Protein, bone morphogenetic protein receptor type 1A Protein, bone morphogenetic protein receptor, type 1A Protein, bone morphogenetic protein receptor type 1A S homeolog Protein, bone morphogenetic protein receptor, type IAa Protein, bone morphogenetic protein receptor, type IAb Protein, BMPR1A Protein, Bmpr1a Protein, bmpr1a Protein, bmpr1a.S Protein, bmpr1aa Protein, bmpr1ab Protein
    Background
    On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP2, BMP4, GDF5 and GDF6. Positively regulates chondrocyte differentiation through GDF5 interaction. Mediates induction of adipogenesis by GDF6. {ECO:0000250|UniProtKB:P36895}.
    Molecular Weight
    41.6 kDa Including tag.
    UniProt
    P36894
    Pathways
    Stem Cell Maintenance
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