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Basonuclin 1 Protein (BNC1) (AA 1-994) (His tag)

Crystallography grade BNC1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3089999
  • Target See all Basonuclin 1 (BNC1) Proteins
    Basonuclin 1 (BNC1)
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-994
    Origin
    • 3
    • 1
    Human
    Source
    • 2
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This Basonuclin 1 protein is labelled with His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    MRRRPPSRGG RGAARARETR RQPRHRSGRR MAEAISCTLN CSCQSFKPGK INHRQCDQCK HGWVAHALSK LRIPPMYPTS QVEIVQSNVV FDISSLMLYG TQAIPVRLKI LLDRLFSVLK QDEVLQILHA LDWTLQDYIR GYVLQDASGK VLDHWSIMTS EEEVATLQQF LRFGETKSIV ELMAIQEKEE QSIIIPPSTA NVDIRAFIES CSHRSSSLPT PVDKGNPSSI HPFENLISNM TFMLPFQFFN PLPPALIGSL PEQYMLEQGH DQSQDPKQEV HGPFPDSSFL TSSSTPFQVE KDQCLNCPDA ITKKEDSTHL SDSSSYNIVT KFERTQLSPE AKVKPERNSL GTKKGRVFCT ACEKTFYDKG TLKIHYNAVH LKIKHKCTIE GCNMVFSSLR SRNRHSANPN PRLHMPMNRN NRDKDLRNSL NLASSENYKC PGFTVTSPDC RPPPSYPGSG EDSKGQPAFP NIGQNGVLFP NLKTVQPVLP FYRSPATPAE VANTPGILPS LPLLSSSIPE QLISNEMPFD ALPKKKSRKS SMPIKIEKEA VEIANEKRHN LSSDEDMPLQ VVSEDEQEAC SPQSHRVSEE QHVQSGGLGK PFPEGERPCH RESVIESSGA ISQTPEQATH NSERETEQTP ALIMVPREVE DGGHEHYFTP GMEPQVPFSD YMELQQRLLA GGLFSALSNR GMAFPCLEDS KELEHVGQHA LARQIEENRF QCDICKKTFK NACSVKIHHK NMHVKEMHTC TVEGCNATFP SRRSRDRHSS NLNLHQKALS QEALESSEDH FRAAYLLKDV AKEAYQDVAF TQQASQTSVI FKGTSRMGSL VYPITQVHSA SLESYNSGPL SEGTILDLST TSSMKSESSS HSSWDSDGVS EEGTVLMEDS DGNCEGSSLV PGEDEYPICV LMEKADQSLA SLPSGLPITC HLCQKTYSNK GTFRAHYKTV HLRQLHKCKV PGCNTMFSSV RSRNRHSQNP NLHKSLASSP SHLQ
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human BNC1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product BNC1 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    Basonuclin 1 (BNC1)
    Alternative Name
    BNC1 (BNC1 Products)
    Synonyms
    fc51b07 Protein, wu:fc51b07 Protein, BNC Protein, BSN1 Protein, HsT19447 Protein, AI047752 Protein, AW546376 Protein, Bnc Protein, basonuclin 1 Protein, BNC1 Protein, bnc1 Protein, Bnc1 Protein
    Background
    Likely to be a transcription factor specific for squamous epithelium and for the constituent keratinocytes at a stage either prior to or at the very beginning of terminal differentiation. May play a role in the differentiation of spermatozoa and oocytes.
    Molecular Weight
    111.9 kDa Including tag.
    UniProt
    Q01954
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