CCDC88B Protein (AA 1-1476) (Strep Tag)

Catalog No. ABIN3090597

Product Details

Target: CCDC88B (Coiled-Coil Domain Containing 88B (CCDC88B))

Protein Type: Recombinant

Protein Characteristics: AA 1-1476

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This CCDC88B protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MEGGKGPRLR DFLSGSLATW ALGLAGLVGE AEDSEGEEEE EEEEPPLWLE KRFLRLSDGA LLLRVLGIIA PSSRGGPRML RGLDGPAAWR VWNLNHLWGR LRDFYQEELQ LLILSPPPDL QTLGFDPLSE EAVEQLEGVL RLLLGASVQC EHRELFIRHI QGLSLEVQSE LAAAIQEVTQ PGAGVVLALS GPDPGELAPA ELEMLSRSLM GTLSKLARER DLGAQRLAEL LLEREPLCLR PEAPSRAPAE GPSHHLALQL ANAKAQLRRL RQELEEKAEL LLDSQAEVQG LEAEIRRLRQ EAQALSGQAK RAELYREEAE ALRERAGRLP RLQEELRRCR ERLQAAEAYK SQLEEERVLS GVLEASKALL EEQLEAARER CARLHETQRE NLLLRTRLGE AHAELDSLRH QVDQLAEENV ELELELQRSL EPPPGSPGEA PLAGAAPSLQ DEVREAEAGR LRTLERENRE LRGLLQVLQG QPGGQHPLLE APREDPVLPV LEEAPQTPVA FDHSPQGLVQ KARDGGPQAL DLAPPALDSV LEASAECPQA PDSDPQEAES PLQAAAMDPQ ASDWSPQESG SPVETQESPE KAGRRSSLQS PASVAPPQGP GTKIQAPQLL GGETEGREAP QGELVPEAWG LRQEGPEHKP GPSEPSSVQL EEQEGPNQGL DLATGQAEAR EHDQRLEGTV RDPAWQKPQQ KSEGALEVQV WEGPIPGESL ASGVAEQEAL REEVAQLRRK AEALGDELEA QARKLEAQNT EAARLSKELA QARRAEAEAH REAEAQAWEQ ARLREAVEAA GQELESASQE REALVEALAA AGRERRQWER EGSRLRAQSE AAEERMQVLE SEGRQHLEEA ERERREKEAL QAELEKAVVR GKELGDRLEH LQRELEQAAL ERQEFLREKE SQHQRYQGLE QRLEAELQAA ATSKEEALME LKTRALQLEE ELFQLRQGPA GLGPKKRAEP QLVETQNVRL IEVERSNAML VAEKAALQGQ LQHLEGQLGS LQGRAQELLL QSQRAQEHSS RLQAEKSVLE IQGQELHRKL EVLEEEVRAA RQSQEETRGQ QQALLRDHKA LAQLQRRQEA ELEGLLVRHR DLKANMRALE LAHRELQGRH EQLQAQRASV EAQEVALLAE RERLMQDGHR QRGLEEELRR LQSEHDRAQM LLAELSRERG ELQGERGELR GRLARLELER AQLEMQSQQL RESNQQLDLS ACRLTTQCEL LTQLRSAQEE ENRQLLAEVQ ALSRENRELL ERSLESRDHL HREQREYLDQ LNALRREKQK LVEKIMDQYR VLEPVPLPRT KKGSWLADKV KRLMRPRREG GPPGGLRLGA DGAGSTESLG GPPETELPEG READGTGSPS PAPMRRAQSS LCLRDETLAG GQRRKLSSRF PVGRSSESFS PGDTPRQRFR QRHPGPLGAP VSHSKGPGVG WENSAETLQE HETDANREGP EVQEPEKRPL TPSLSQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: CCDC88B (Coiled-Coil Domain Containing 88B (CCDC88B))

Alternative Name: CCDC88B (CCDC88B Products)

Synonyms: ccdc88a Protein, MGC175202 Protein, 2610041P08Rik Protein, Ccdc88 Protein, BRLZ Protein, CCDC88 Protein, HKRP3 Protein, gipie Protein, RGD1311475 Protein, coiled-coil domain containing 88B Protein, ccdc88b Protein, Ccdc88b Protein, CCDC88B Protein

Background: Coiled-coil domain-containing protein 88B (Brain leucine zipper domain-containing protein) (Gipie) (Hook-related protein 3) (HkRP3),FUNCTION: Acts as a positive regulator of T-cell maturation and inflammatory function. Required for several functions of T-cells, in both the CD4(+) and the CD8(+) compartments and this includes expression of cell surface markers of activation, proliferation, and cytokine production in response to specific or non-specific stimulation (By similarity). Enhances NK cell cytotoxicity by positively regulating polarization of microtubule-organizing center (MTOC) to cytotoxic synapse, lytic granule transport along microtubules, and dynein-mediated clustering to MTOC (PubMed:25762780). Interacts with HSPA5 and stabilizes the interaction between HSPA5 and ERN1, leading to suppression of ERN1-induced JNK activation and endoplasmic reticulum stress-induced apoptosis (PubMed:21289099). {ECO:0000250|UniProtKB:Q4QRL3, ECO:0000269|PubMed:21289099, ECO:0000269|PubMed:25762780}.

Molecular Weight: 164.8 kDa

UniProt: A6NC98