OB Cadherin Protein (AA 54-617) (MBP tag)

Catalog No. ABIN3090705

Product Details

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Protein Type: Recombinant

Protein Characteristics: AA 54-617

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This OB Cadherin protein is labelled with MBP tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequence: GWVWNQFFVI EEYTGPDPVL VGRLHSDIDS GDGNIKYILS GEGAGTIFVI DDKSGNIHAT KTLDREERAQ YTLMAQAVDR DTNRPLEPPS EFIVKVQDIN DNPPEFLHET YHANVPERSN VGTSVIQVTA SDADDPTYGN SAKLVYSILE GQPYFSVEAQ TGIIRTALPN MDREAKEEYH VVIQAKDMGG HMGGLSGTTK VTITLTDVND NPPKFPQSVY QMSVSEAAVP GEEVGRVKAK DPDIGENGLV TYNIVDGDGM ESFEITTDYE TQEGVIKLKK PVDFETKRAY SLKVEAANVH IDPKFISNGP FKDTVTVKIS VEDADEPPMF LAPSYIHEVQ ENAAAGTVVG RVHAKDPDAA NSPIRYSIDR HTDLDRFFTI NPEDGFIKTT KPLDREETAW LNITVFAAEI HNRHQEAKVP VAIRVLDVND NAPKFAAPYE GFICESDQTK PLSNQPIVTI SADDKDDTAN GPRFIFSLPP EIIHNPNFTV RDNRDNTAGV YARRGGFSRQ KQDLYLLPIV ISDGGIPPMS STNTLTIKVC GCDVNGALLS CNAEAYILNA GLST
Sequence without tag.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human CDH11 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: The protein is purified from the cleared cell lysate using MBP capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

N-terminal MBP-Tag

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: in solution (20 mM Hepes, pH 7.4, 5 mM NaCl)

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: OB Cadherin (CDH11) (Cadherin 11 (CDH11))

Alternative Name: CDH11 (CDH11 Products)

Synonyms: cad11 Protein, cdhob Protein, osf-4 Protein, Xcad-11 Protein, MGC108014 Protein, OB-cadherin Protein, cadherin-11 Protein, Cad11 Protein, cdhvn Protein, vnc Protein, CAD11 Protein, CDHOB Protein, OB Protein, OSF-4 Protein, cadherin 11 Protein, cadherin 11, type 2, OB-cadherin (osteoblast) Protein, cadherin 11 S homeolog Protein, CDH11 Protein, cdh11 Protein, Cdh11 Protein, cdh11.S Protein

Background: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells, cadherins may thus contribute to the sorting of heterogeneous cell types.

Molecular Weight: 62.9 kDa Including tag.

UniProt: P55287