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AGBL1 Protein (AA 1-1066) (His tag)

Crystallography grade AGBL1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. Crys, ELISA, SDS, WB
Catalog No. ABIN3090935
  • Target See all AGBL1 products
    AGBL1 (ATP/GTP Binding Protein-Like 1 (AGBL1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-1066
    Origin
    Human
    Source
    • 1
    Insect Cells
    Purification tag / Conjugate
    This AGBL1 protein is labelled with His tag.
    Application
    Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
    Sequence
    MISKGGSEAL LQTLVDTART APPDYDILLP LFRLLAKVGL RDKKIGRKAL ELEALDVTLI LARKNLSHGQ NLLHCLWALR VFASSVSMGA MLGINGAMEL LFKVITPYTR KRTQAIRAAT EVLAALLKSK SNGRRAVNRG YVTSLLGLHQ DWHSHDTANA YVQIRRGLLL CLRHIAALRS GREAFLAAQG MEILFSTTQN CLDDKSMEPV ISVVLQILRQ CYPTSPLPLV TASSAYAFPV PGCITTEPPH DLPEEDFEDD GDDEVDKDSD TEDGKVEDDD LETDVNKLSS KPGLDRPEEE LMQYEVMCLE LSYSFEELQS KLGDDLNSEK TQYANHHHIP AAASSKQHCY SKDQSSCGQE REYAVQTSLL CRVKTGRSTV HLGSKKNPGV NLYQNVQSNS LRRDSSESEI PDIQASPKAD AWDVDAIFCP RMSASFSNST RTREVVKVID KLLQTHLKRV PFHDPYLYMA KARRTSSVVD FKMMAFPDVW GHCPPPTTQP MLERKCGVQR IRIFEDIRRL IQPSDVINKV VFSLDEPWPL QDNASNCLRF FSKFESGNLR KAIQVREFEY DLLVNADVNS TQHQQWFYFK VSGMQAAIPY HFNIINCEKP NSQFNYGMQP TLYSVKEALL GKPTWIRTGH EICYYKNHYR QSTAVAGGAS GKCYYTLTFA VTFPHSEDVC YLAYHYPYTY TALMTHLDIL EKSVNLKEVY FRQDVLCQTL GGNPCPLVTI TAMPESNSDE HLEQFRHRPY QVITARVHPG ESNASWVMKG TLEFLVSSDP VARLLRENFI FKIIPMLNPD GVINGNHRCS LSGEDLNRQW LSPSAHLQPT IYHAKGLLYH LSSIGRSPVV FCDFHGHSQK KNVFLYGCSI KETLWQAACT VGTSTILEEV NYRTLPKILD KLAPAFTMSS CSFLVEKSRA STARVVVWRE MGVSRSYTME SSYCGCNQGP YQCTQRLLER TKNERAHPVD GLQGLQFGTR ELEEMGAMFC LGLLILELKS ASCSHQLLAQ AATLLSAEED ALDQHLQRLK SSNFLPKHIW FAYHFFAITN FFKMNLLLHV SPVCDT
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human AGBL1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    AGBL1 (ATP/GTP Binding Protein-Like 1 (AGBL1))
    Alternative Name
    AGBL1 (AGBL1 Products)
    Synonyms
    RGD1560280 Protein, Ccp4 Protein, D430020F16 Protein, EG244071 Protein, Nna1-l1 Protein, ATP/GTP binding protein-like 1 Protein, ATP/GTP binding protein like 1 Protein, cytosolic carboxypeptidase 4 Protein, Agbl1 Protein, AGBL1 Protein, LOC100300175 Protein, agbl1 Protein
    Background
    Metallocarboxypeptidase that mediates deglutamylation of target proteins. Catalyzes the deglutamylation of polyglutamate side chains generated by post-translational polyglutamylation in proteins such as tubulins. Also removes gene-encoded polyglutamates from the carboxy-terminus of target proteins such as MYLK. Acts as a long-chain deglutamylase and specifically shortens long polyglutamate chains, while it is not able to remove the branching point glutamate, a process catalyzed by AGBL5/CCP5. {ECO:0000250|UniProtKB:Q09M05}.
    Molecular Weight
    121.2 kDa Including tag.
    UniProt
    Q96MI9
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