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CNOT3 Protein (AA 1-753) (His tag)

Crystallography grade CNOT3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3091878
  • Target See all CNOT3 Proteins
    CNOT3 (CCR4-NOT Transcription Complex, Subunit 3 (CNOT3))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-753
    Origin
    • 2
    • 1
    Human
    Source
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This CNOT3 protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    MADKRKLQGE IDRCLKKVSE GVEQFEDIWQ KLHNAANANQ KEKYEADLKK EIKKLQRLRD QIKTWVASNE IKDKRQLIDN RKLIETQMER FKVVERETKT KAYSKEGLGL AQKVDPAQKE KEEVGQWLTN TIDTLNMQVD QFESEVESLS VQTRKKKGDK DKQDRIEGLK RHIEKHRYHV RMLETILRML DNDSILVDAI RKIKDDVEYY VDSSQDPDFE ENEFLYDDLD LEDIPQALVA TSPPSHSHME DEIFNQSSST PTSTTSSSPI PPSPANCTTE NSEDDKKRGR STDSEVSQSP AKNGSKPVHS NQHPQSPAVP PTYPSGPPPA ASALSTTPGN NGVPAPAAPP SALGPKASPA PSHNSGTPAP YAQAVAPPAP SGPSTTQPRP PSVQPSGGGG GGSGGGGSSS SSNSSAGGGA GKQNGATSYS SVVADSPAEV ALSSSGGNNA SSQALGPPSG PHNPPPSTSK EPSAAAPTGA GGVAPGSGNN SGGPSLLVPL PVNPPSSPTP SFSDAKAAGA LLNGPPQFST APEIKAPEPL SSLKSMAERA AISSGIEDPV PTLHLTERDI ILSSTSAPPA SAQPPLQLSE VNIPLSLGVC PLGPVPLTKE QLYQQAMEEA AWHHMPHPSD SERIRQYLPR NPCPTPPYHH QMPPPHSDTV EFYQRLSTET LFFIFYYLEG TKAQYLAAKA LKKQSWRFHT KYMMWFQRHE EPKTITDEFE QGTYIYFDYE KWGQRKKEGF TFEYRYLEDR DLQ
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human CNOT3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product CNOT3 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    CNOT3 (CCR4-NOT Transcription Complex, Subunit 3 (CNOT3))
    Alternative Name
    CNOT3 (CNOT3 Products)
    Synonyms
    LENG2 Protein, NOT3 Protein, NOT3H Protein, A930039N10Rik Protein, cnot3 Protein, zgc:55934 Protein, wu:fi20g07 Protein, fb73b06 Protein, fc17h07 Protein, wu:fb73b06 Protein, wu:fc17h07 Protein, zgc:92813 Protein, CNOT3 Protein, Afu2g02070 Protein, AO090011000479 Protein, CG8426 Protein, Dmel\\CG8426 Protein, NOT3.5 Protein, NOT3/5 Protein, Not3 Protein, not3 Protein, not3/5 Protein, leng2 Protein, not3h Protein, CCR4-NOT transcription complex subunit 3 Protein, CCR4-NOT transcription complex, subunit 3 Protein, CCR4-NOT transcription complex, subunit 3b Protein, CCR4-NOT transcription complex, subunit 3a Protein, CCR4-NOT transcription complex subunit 3 S homeolog Protein, CNOT3 Protein, Cnot3 Protein, cnot3b Protein, cnot3a Protein, AFUA_2G02070 Protein, NFIA_034440 Protein, ACLA_092760 Protein, PMAA_089540 Protein, AFLA_043060 Protein, TSTA_125290 Protein, ANI_1_738064 Protein, AOR_1_812054 Protein, Not3 Protein, cnot3 Protein, cnot3.S Protein
    Background
    Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation, may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation, the repressive function may involve histone deacetylases. Involved in the maintenance of emryonic stem (ES) cell identity. {ECO:0000269|PubMed:14707134, ECO:0000269|PubMed:22342980, ECO:0000269|PubMed:22367759}.
    Molecular Weight
    82.8 kDa Including tag.
    UniProt
    O75175
    Pathways
    Stem Cell Maintenance
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