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CYP3A4 Protein (AA 2-503) (rho-1D4 tag)

Crystallography grade CYP3A4 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3091896
  • Target See all CYP3A4 Proteins
    CYP3A4 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 2-503
    Origin
    • 9
    • 2
    Human
    Source
    • 5
    • 3
    • 2
    • 1
    Insect Cells
    Purification tag / Conjugate
    This CYP3A4 protein is labelled with rho-1D4 tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    ALIPDLAMET WLLLAVSLVL LYLYGTHSHG LFKKLGIPGP TPLPFLGNIL SYHKGFCMFD MECHKKYGKV WGFYDGQQPV LAITDPDMIK TVLVKECYSV FTNRRPFGPV GFMKSAISIA EDEEWKRLRS LLSPTFTSGK LKEMVPIIAQ YGDVLVRNLR REAETGKPVT LKDVFGAYSM DVITSTSFGV NIDSLNNPQD PFVENTKKLL RFDFLDPFFL SITVFPFLIP ILEVLNICVF PREVTNFLRK SVKRMKESRL EDTQKHRVDF LQLMIDSQNS KETESHKALS DLELVAQSII FIFAGYETTS SVLSFIMYEL ATHPDVQQKL QEEIDAVLPN KAPPTYDTVL QMEYLDMVVN ETLRLFPIAM RLERVCKKDV EINGMFIPKG VVVMIPSYAL HRDPKYWTEP EKFLPERFSK KNKDNIDPYI YTPFGSGPRN CIGMRFALMN MKLALIRVLQ NFSFKPCKET QIPLKLSLGG LLQPEKPVVL KVESRDGTVS GA
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human CYP3A4 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin-free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product CYP3A4 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    CYP3A4 (Cytochrome P450, Family 3, Subfamily A, Polypeptide 4 (CYP3A4))
    Alternative Name
    CYP3A4 (CYP3A4 Products)
    Synonyms
    CP33 Protein, CP34 Protein, CYP3A Protein, CYP3A3 Protein, CYPIIIA3 Protein, CYPIIIA4 Protein, HLP Protein, NF-25 Protein, P450C3 Protein, P450PCN1 Protein, MGC108372 Protein, CYP3A80 Protein, CYP3A4 Protein, CYP3A21 Protein, CYPIIIA21 Protein, CYP3A12 Protein, cytochrome P450 family 3 subfamily A member 4 Protein, cytochrome P450 family 3 subfamily A member 43 Protein, cytochrome P450 3A4 Protein, cytochrome P450, subfamily IIIA, polypeptide 4 Protein, cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4 Protein, cytochrome P450, family 3, subfamily A, polypeptide 4 Protein, CYP3A4 Protein, CYP3A43 Protein, PTRG_01782 Protein, PTRG_06060 Protein, cyp3a4 Protein
    Background
    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It performs a variety of oxidation reactions (e.g. caffeine 8-oxidation, omeprazole sulphoxidation, midazolam 1'-hydroxylation and midazolam 4-hydroxylation) of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. Acts as a 1,8-cineole 2-exo-monooxygenase. The enzyme also hydroxylates etoposide (PubMed:11159812). Catalyzes 4-beta-hydroxylation of cholesterol. May catalyze 25-hydroxylation of cholesterol in vitro (PubMed:21576599). {ECO:0000269|PubMed:11159812, ECO:0000269|PubMed:21576599}.
    Molecular Weight
    58.4 kDa Including tag.
    UniProt
    P08684
    Pathways
    Steroid Hormone Biosynthesis
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