Corin Protein (AA 67-1042) (His tag)

Catalog No. ABIN3091927

Product Details

Target: Corin (CORIN) (Corin, Serine Peptidase (CORIN))

Protein Type: Recombinant

Protein Characteristics: AA 67-1042

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This Corin protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: SYVGTLQKVY FKSNGSEPLV TDGEIQGSDV ILTNTIYNQS TVVSTAHPDQ HVPAWTTDAS LPGDQSHRNT SACMNITHSQ CQMLPYHATL TPLLSVVRNM EMEKFLKFFT YLHRLSCYQH IMLFGCTLAF PECIIDGDDS HGLLPCRSFC EAAKEGCESV LGMVNYSWPD FLRCSQFRNQ TESSNVSRIC FSPQQENGKQ LLCGRGENFL CASGICIPGK LQCNGYNDCD DWSDEAHCNC SENLFHCHTG KCLNYSLVCD GYDDCGDLSD EQNCDCNPTT EHRCGDGRCI AMEWVCDGDH DCVDKSDEVN CSCHSQGLVE CRNGQCIPST FQCDGDEDCK DGSDEENCSV IQTSCQEGDQ RCLYNPCLDS CGGSSLCDPN NSLNNCSQCE PITLELCMNL PYNSTSYPNY FGHRTQKEAS ISWESSLFPA LVQTNCYKYL MFFSCTILVP KCDVNTGEHI PPCRALCEHS KERCESVLGI VGLQWPEDTD CSQFPEENSD NQTCLMPDEY VEECSPSHFK CRSGQCVLAS RRCDGQADCD DDSDEENCGC KERDLWECPS NKQCLKHTVI CDGFPDCPDY MDEKNCSFCQ DDELECANHA CVSRDLWCDG EADCSDSSDE WDCVTLSINV NSSSFLMVHR AATEHHVCAD GWQEILSQLA CKQMGLGEPS VTKLIQEQEK EPRWLTLHSN WESLNGTTLH ELLVNGQSCE SRSKISLLCT KQDCGRRPAA RMNKRILGGR TSRPGRWPWQ CSLQSEPSGH ICGCVLIAKK WVLTVAHCFE GRENAAVWKV VLGINNLDHP SVFMQTRFVK TIILHPRYSR AVVDYDISIV ELSEDISETG YVRPVCLPNP EQWLEPDTYC YITGWGHMGN KMPFKLQEGE VRIISLEHCQ SYFDMKTITT RMICAGYESG TVDSCMGDSG GPLVCEKPGG RWTLFGLTSW GSVCFSKVLG PGVYSNVSYF VEWIKRQIYI QTFLLN
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human CORIN Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Corin (CORIN) (Corin, Serine Peptidase (CORIN))

Alternative Name: CORIN (CORIN Products)

Synonyms: CORIN Protein, CG2105 Protein, Dmel\\CG2105 Protein, SP75 Protein, LOC559109 Protein, AV273130 Protein, Lrp4 Protein, ATC2 Protein, CRN Protein, PEE5 Protein, TMPRSS10 Protein, corin, serine peptidase Protein, CG2105 gene product from transcript CG2105-RC Protein, novel protein similar to H.sapiens CORIN, corin, serine peptidase (CORIN) Protein, corin Protein, CORIN Protein, Corin Protein, LOC559109 Protein

Background: Serine-type endopeptidase involved in atrial natriuretic peptide hormone (NPPA) processing. Converts through proteolytic cleavage the non-functional propeptide NPPA into the active hormone, thereby regulating blood pressure in heart and promoting natriuresis, diuresis and vasodilation. Proteolytic cleavage of pro-NPPA also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Also acts as a regulator of sodium reabsorption in kidney. May also process pro-NPPB the B-type natriuretic peptide., Isoform 2: has weaker endopeptidase activity compared to isoform 1.

Molecular Weight: 110.3 kDa Including tag.

UniProt: Q9Y5Q5

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones