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GPS1 Protein (AA 2-491) (His tag)

Crystallography grade GPS1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3091964
  • Target See all GPS1 Proteins
    GPS1 (G Protein Pathway Suppressor 1 (GPS1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 2-491
    Origin
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Source
    • 4
    • 2
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This GPS1 protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    PLPVQVFNLQ GAVEPMQIDV DPQEDPQNAP DVNYVVENPS LDLEQYAASY SGLMRIERLQ FIADHCPTLR VEALKMALSF VQRTFNVDMY EEIHRKLSEA TRSSLRELQN APDAIPESGV EPPALDTAWV EATRKKALLK LEKLDTDLKN YKGNSIKESI RRGHDDLGDH YLDCGDLSNA LKCYSRARDY CTSAKHVINM CLNVIKVSVY LQNWSHVLSY VSKAESTPEI AEQRGERDSQ TQAILTKLKC AAGLAELAAR KYKQAAKCLL LASFDHCDFP ELLSPSNVAI YGGLCALATF DRQELQRNVI SSSSFKLFLE LEPQVRDIIF KFYESKYASC LKMLDEMKDN LLLDMYLAPH VRTLYTQIRN RALIQYFSPY VSADMHRMAA AFNTTVAALE DELTQLILEG LISARVDSHS KILYARDVDQ RSTTFEKSLL MGKEFQRRAK AMMLRAAVLR NQIHVKSPPR EGSQGELTPA NSQSRMSTNM
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human GPS1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product GPS1 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    GPS1 (G Protein Pathway Suppressor 1 (GPS1))
    Alternative Name
    GPS1 (GPS1 Products)
    Synonyms
    COPS1 Protein, CSN1 Protein, Cops1 Protein, Csn1 Protein, R75577 Protein, Sgn1 Protein, im:6909260 Protein, im:7145572 Protein, zgc:154082 Protein, csn1 Protein, ARABIDOPSIS THALIANA FUSCA 6 Protein, ATFUS6 Protein, ATSK31 Protein, CONSTITUTIVE PHOTOMORPHOGENIC 11 Protein, COP11 Protein, COP9 SIGNALOSOME SUBUNIT 1 Protein, EMB78 Protein, EMBRYO DEFECTIVE 78 Protein, FUSCA 6 Protein, SHAGGY-LIKE KINASE 31 Protein, SK31 Protein, G protein pathway suppressor 1 Protein, casein alpha s1 Protein, G protein pathway suppressor 1 L homeolog Protein, COP9 signalosome complex subunit 1 Protein, GPS1 Protein, CSN1S1 Protein, LOC693104 Protein, CC1G_07518 Protein, Bm1_55055 Protein, Gps1 Protein, gps1 Protein, gps1.L Protein, FUS6 Protein, csn-1 Protein
    Background
    Essential component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. Suppresses G-protein- and mitogen-activated protein kinase-mediated signal transduction. {ECO:0000269|PubMed:11285227, ECO:0000269|PubMed:11337588, ECO:0000269|PubMed:12628923, ECO:0000269|PubMed:12732143, ECO:0000269|PubMed:9535219}.
    Molecular Weight
    56.4 kDa Including tag.
    UniProt
    Q13098
    Pathways
    Cell Division Cycle
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