COPS3 Protein (AA 2-423) (His tag)

Catalog No. ABIN3091994

Product Details

Target: COPS3 (COP9 Signalosome Complex Subunit 3 (COPS3))

Protein Type: Recombinant

Protein Characteristics: AA 2-423

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This COPS3 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: ASALEQFVNS VRQLSAQGQM TQLCELINKS GELLAKNLSH LDTVLGALDV QEHSLGVLAV LFVKFSMPSV PDFETLFSQV QLFISTCNGE HIRYATDTFA GLCHQLTNAL VERKQPLRGI GILKQAIDKM QMNTNQLTSI HADLCQLCLL AKCFKPALPY LDVDMMDICK ENGAYDAKHF LCYYYYGGMI YTGLKNFERA LYFYEQAITT PAMAVSHIML ESYKKYILVS LILLGKVQQL PKYTSQIVGR FIKPLSNAYH ELAQVYSTNN PSELRNLVNK HSETFTRDNN MGLVKQCLSS LYKKNIQRLT KTFLTLSLQD MASRVQLSGP QEAEKYVLHM IEDGEIFASI NQKDGMVSFH DNPEKYNNPA MLHNIDQEML KCIELDERLK AMDQEITVNP QFVQKSMGSQ EDDSGNKPSS YS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human COPS3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: COPS3 (COP9 Signalosome Complex Subunit 3 (COPS3))

Alternative Name: COPS3 (COPS3 Products)

Synonyms: CG18332 Protein, CH3 Protein, Csn3 Protein, DCH3 Protein, Dch3 Protein, Dmel\\CG18332 Protein, FBgn0027055 Protein, dCSN3 Protein, COPS3 Protein, CSN3 Protein, DKFZp468P1922 Protein, Sgn3 Protein, wu:fc32a02 Protein, wu:fk59f01 Protein, zgc:111903 Protein, zgc:55643 Protein, zgc:77615 Protein, CONSTITUTIVE PHOTOMORPHOGENIC 13 Protein, COP9 SIGNALOSOME SUBUNIT 3 Protein, F18O22.40 Protein, F18O22_40 Protein, FUS11 Protein, FUSCA 11 Protein, SGN3 Protein, csn3 Protein, COP9 signalosome subunit 3 Protein, Proteasome component (PCI) domain protein Protein, COP9 signalosome subunit 3 L homeolog Protein, COP9 signalosome complex subunit 3 Protein, CSN3 Protein, cops3 Protein, COPS3 Protein, Cops3 Protein, COP13 Protein, cops3.L Protein, csn-3 Protein

Background: Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF-type complexes such as SCF, CSA or DDB2. The complex is also involved in phosphorylation of p53/TP53, c-jun/JUN, IkappaBalpha/NFKBIA, ITPK1 and IRF8/ICSBP, possibly via its association with CK2 and PKD kinases. CSN-dependent phosphorylation of TP53 and JUN promotes and protects degradation by the Ubl system, respectively. {ECO:0000269|PubMed:11285227, ECO:0000269|PubMed:11337588, ECO:0000269|PubMed:12628923, ECO:0000269|PubMed:12732143, ECO:0000269|PubMed:9535219}.

Molecular Weight: 48.7 kDa Including tag.

UniProt: Q9UNS2

Pathways: Cell Division Cycle