DACT2 Protein (AA 1-774) (Strep Tag)

Catalog No. ABIN3092015

Product Details

Target: DACT2 (Dapper, Antagonist of beta-Catenin 2 (DACT2))

Protein Type: Recombinant

Protein Characteristics: AA 1-774

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This DACT2 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MWTPGGPPGS AGWDRRRLGA RLRAAFAGLQ ELQGLRATQQ ERVRGALALQ PPPAPAAPCG PHGLHGPEQQ LEAALAALQE QLSRLRQQDI GLKTHLDQLD LQISKLQLDV GTASGEALDS DSRPSSGFYE MSDGGSCSLS TSCASVCSDH ISPSLGSLLP VAQAHKARPS MGDWRPRSVD ETTVPAWRPQ ATEEGARPPG SVEDAGQPWG TFWPRPVSTG DLDRALPADT GLQKASADAE LLGLLCQGVD IPLHVPDPKY RQDLVSQGGR EVYPYPSPLH AVALQSPLFV LTKETPQRGG PSFPRESPRG PAGLNTIQTG PVLEAGPARA RAYIDRLLHL WGRETPAKGS EGEQGPLRHA ASPSPQRQGG WSTDGGGRLL VFAPGREDEG GPAQSRGAGR GGPQQQGYMP LEGPQQSGSL PEEGSKPSNS CVLRETMVQA SPSSKAQQTP SAQDYGRGNI ISPSRMLDKS PSPASGHFAH PSFAASLKMG PPKSKAEKIK RSPMDKVLRF ARQPLLLLDR PEGAHAAPQP SLEWDPAHWP TGRGGLQRRP ALAWEAPGRS CSESTLYPMP VLVPLAVAPQ ESHRTSAQAL FPFEASLLTS VARRKHRRWQ STVEISARAR LASCPESNLG PPRPVARRAG GPLARGRPSL VRQDAYTRSD SEPSKHSAEC DPRFPSVIPE TSEGESSDHT TNRFGDRESS SSDEEGGAQS RDCDLALGYV AAGHAELAWT QEAPVSSGPL LSPVPKLCRI KASKALKKKI RRFQPTALKV MTMV
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: DACT2 (Dapper, Antagonist of beta-Catenin 2 (DACT2))

Alternative Name: DACT2 (DACT2 Products)

Synonyms: 2900084M21Rik Protein, A630024E20 Protein, Dpr2 Protein, Frd2 Protein, dapper2 Protein, C6orf116 Protein, DAPPER2 Protein, DPR2 Protein, RP11-503C24.7 Protein, bA503C24.7 Protein, dishevelled binding antagonist of beta catenin 2 Protein, dishevelled-binding antagonist of beta-catenin 2 Protein, DACT2 Protein, Dact2 Protein

Background: Dapper homolog 2 (Dapper antagonist of catenin 2),FUNCTION: Involved in regulation of intracellular signaling pathways during development. Negatively regulates the Nodal signaling pathway, possibly by promoting the lysosomal degradation of Nodal receptors, such as TGFBR1. May be involved in control of the morphogenetic behavior of kidney ureteric bud cells by keeping cells epithelial and restraining their mesenchymal character. May play an inhibitory role in the re-epithelialization of skin wounds by attenuating TGF-beta signaling (By similarity). {ECO:0000250}.

Molecular Weight: 82.7 kDa

UniProt: Q5SW24