DIP2B Protein (AA 1-1576) (His tag)
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- Target See all DIP2B products
- DIP2B (DIP2 Disco-Interacting Protein 2 Homolog B (DIP2B))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1576
- Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This DIP2B protein is labelled with His tag.
- Application
- Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
- Sequence
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MAERGLEPSP AAVAALPPEV RAQLAELELE LSEGDITQKG YEKKRSKLLS PYSPQTQETD SAVQKELRNQ TPAPSAAQTS APSKYHRTRS GGARDERYRS DIHTEAVQAA LAKHKEQKMA LPMPTKRRST FVQSPADACT PPDTSSASED EGSLRRQAAL SAALQQSLQN AESWINRSIQ GSSTSSSASS TLSHGEVKGT SGSLADVFAN TRIENFSAPP DVTTTTSSSS SSSSIRPANI DLPPSGIVKG MHKGSNRSSL MDTADGVPVS SRVSTKIQQL LNTLKRPKRP PLKEFFVDDS EEIVEVPQPD PNQPKPEGRQ MTPVKGEPLG VICNWPPALE SALQRWGTTQ AKCSCLTALD MTGKPVYTLT YGKLWSRSLK LAYTLLNKLG TKNEPVLKPG DRVALVYPNN DPVMFMVAFY GCLLAEVIPV PIEVPLTRKD AGGQQIGFLL GSCGIALALT SEVCLKGLPK TQNGEIVQFK GWPRLKWVVT DSKYLSKPPK DWQPHISPAG TEPAYIEYKT SKEGSVMGVT VSRLAMLSHC QALSQACNYS EGETIVNVLD FKKDAGLWHG MFANVMNKMH TISVPYSVMK TCPLSWVQRV HAHKAKVALV KCRDLHWAMM AHRDQRDVSL SSLRMLIVTD GANPWSVSSC DAFLSLFQSH GLKPEAICPC ATSAEAMTVA IRRPGVPGAP LPGRAILSMN GLSYGVIRVN TEDKNSALTV QDVGHVMPGG MMCIVKPDGP PQLCKTDEIG EICVSSRTGG MMYFGLAGVT KNTFEVIPVN SAGSPVGDVP FIRSGLLGFV GPGSLVFVVG KMDGLLMVSG RRHNADDIVA TGLAVESIKT VYRGRIAVFS VSVFYDERIV VVAEQRPDAS EEDSFQWMSR VLQAIDSIHQ VGVYCLALVP ANTLPKTPLG GIHISQTKQL FLEGSLHPCN ILMCPHTCVT NLPKPRQKQP GVGPASVMVG NLVAGKRIAQ AAGRDLGQIE ENDLVRKHQF LAEILQWRAQ ATPDHVLFML LNAKGTTVCT ASCLQLHKRA ERIASVLGDK GHLNAGDNVV LLYPPGIELI AAFYGCLYAG CIPVTVRPPH AQNLTATLPT VRMIVDVSKA ACILTSQTLM RLLRSREAAA AVDVKTWPTI IDTDDLPRKR LPQLYKPPTP EMLAYLDFSV STTGMLTGVK MSHSAVNALC RAIKLQCELY SSRQIAICLD PYCGLGFALW CLCSVYSGHQ SVLIPPMELE NNLFLWLSTV NQYKIRDTFC SYSVMELCTK GLGNQVEVLK TRGINLSCVR TCVVVAEERP RVALQQSFSK LFKDIGLSPR AVSTTFGSRV NVAICLQGTS GPDPTTVYVD LKSLRHDRVR LVERGAPQSL LLSESGKILP GVKVVIVNPE TKGPVGDSHL GEIWVNSPHT ASGYYTIYDS ETLQADHFNT RLSFGDAAQT LWARTGYLGF VRRTELTAAT GERHDALYVV GALDETLELR GLRYHPIDIE TSVSRIHRSI AECAVFTWTN LLVVVVELCG SEQEALDLVP LVTNVVLEEH YLIVGVVVVV DPGVIPINSR GEKQRMHLRD SFLADQLDPI YVAYNM
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human DIP2B Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- DIP2B (DIP2 Disco-Interacting Protein 2 Homolog B (DIP2B))
- Alternative Name
- DIP2B (DIP2B Products)
- Synonyms
- RGD1305671 Protein, dip2b Protein, zgc:77348 Protein, 4932422C22 Protein, AI317237 Protein, AI854602 Protein, mKIAA1463 Protein, disco-interacting protein 2 homolog B Protein, disco-interacting protein 2 homolog Ba Protein, disco interacting protein 2 homolog B Protein, Dip2b Protein, dip2ba Protein, DIP2B Protein, dip2b Protein
- Molecular Weight
- 172.4 kDa Including tag.
- UniProt
- Q9P265
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