DIP2A Protein (AA 1-1571) (Strep Tag)

Catalog No. ABIN3092160

Product Details

Target: DIP2A (DIP2 Disco-Interacting Protein 2 Homolog A (DIP2A))

Protein Type: Recombinant

Protein Characteristics: AA 1-1571

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This DIP2A protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MADRGCPLEA APLPAEVRES LAELELELSE GDITQKGYEK KRAKLLARYI PLIQGIDPSL QAENRIPGPS QTTAAAPKQQ KSRPTASRDE RFRSDVHTEA VQAALAKYKE RKMPMPSKRR SVLVHSSVET YTPPDTSSAS EDEGSLRRPG RLTSTPLQSH SSVEPWLDRV IQGSSTSSSA SSTSSHPGGR PTTAPSAAAT PGAAATTALA GLEAHTHIDL HSAPPDVTTG LVEHSYFERP QVASVRSVPR GCSGSMLETA DGVPVNSRVS SKIQQLLNTL KRPKRPPLKE FFVDDFEELL EVQQPDPNQP KPEGSETSVL RGEPLTAGVP RPPSLLATLQ RWGTTQPKSP CLTALDTTGK AVYTLTYGKL WSRSLKLAYT LLNKLTSKNE PLLKPGDRVA LVFPNSDPVM FMVAFYGCLL AELVPVPIEV PLTRKDAGSQ QVGFLLGSCG VFLALTTDAC QKGLPKAQTG EVAAFKGWPP LSWLVIDGKH LAKPPKDWHP LAQDTGTGTA YIEYKTSKEG STVGVTVSHA SLLAQCRALT QACGYSEAET LTNVLDFKRD AGLWHGVLTS VMNRMHVVSV PYALMKANPL SWIQKVCFYK ARAALVKSRD MHWSLLAQRG QRDVSLSSLR MLIVADGANP WSISSCDAFL NVFQSRGLRP EVICPCASSP EALTVAIRRP PDLGGPPPRK AVLSMNGLSY GVIRVDTEEK LSVLTVQDVG QVMPGANVCV VKLEGTPYLC KTDEVGEICV SSSATGTAYY GLLGITKNVF EAVPVTTGGA PIFDRPFTRT GLLGFIGPDN LVFIVGKLDG LMVTGVRRHN ADDVVATALA VEPMKFVYRG RIAVFSVTVL HDDRIVLVAE QRPDASEEDS FQWMSRVLQA IDSIHQVGVY CLALVPANTL PKAPLGGIHI SETKQRFLEG TLHPCNVLMC PHTCVTNLPK PRQKQPEVGP ASMIVGNLVA GKRIAQASGR ELAHLEDSDQ ARKFLFLADV LQWRAHTTPD HPLFLLLNAK GTVTSTATCV QLHKRAERVA AALMEKGRLS VGDHVALVYP PGVDLIAAFY GCLYCGCVPV TVRPPHPQNL GTTLPTVKMI VEVSKSACVL TTQAVTRLLR SKEAAAAVDI RTWPTILDTD DIPKKKIASV FRPPSPDVLA YLDFSVSTTG ILAGVKMSHA ATSALCRSIK LQCELYPSRQ IAICLDPYCG LGFALWCLCS VYSGHQSVLV PPLELESNVS LWLSAVSQYK ARVTFCSYSV MEMCTKGLGA QTGVLRMKGV NLSCVRTCMV VAEERPRIAL TQSFSKLFKD LGLPARAVST TFGCRVNVAI CLQGTAGPDP TTVYVDMRAL RHDRVRLVER GSPHSLPLME SGKILPGVKV IIAHTETKGP LGDSHLGEIW VSSPHNATGY YTVYGEEALH ADHFSARLSF GDTQTIWART GYLGFLRRTE LTDASGGRHD ALYVVGSLDE TLELRGMRYH PIDIETSVIR AHRSIAECAV FTWTNLLVVV VELDGLEQDA LDLVALVTNV VLEEHYLVVG VVVIVDPGVI PINSRGEKQR MHLRDGFLAD QLDPIYVAYN M
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: DIP2A (DIP2 Disco-Interacting Protein 2 Homolog A (DIP2A))

Alternative Name: DIP2A (DIP2A Products)

Synonyms: C21orf106 Protein, Dip2-like Protein, DIP2 Protein, 4931420H10Rik Protein, AI426328 Protein, Dip2 Protein, Kiaa0184-hp Protein, mKIAA0184 Protein, disco-interacting protein 2 homolog A Protein, disco interacting protein 2 homolog A Protein, Dip2a Protein, DIP2A Protein

Background: Disco-interacting protein 2 homolog A (DIP2 homolog A) (EC 6.2.1.1),FUNCTION: Catalyzes the de novo synthesis of acetyl-CoA in vitro (By similarity). Promotes acetylation of CTTN, possibly by providing the acetyl donor, ensuring correct dendritic spine morphology and synaptic transmission (By similarity). Binds to follistatin-related protein FSTL1 and may act as a cell surface receptor for FSTL1, contributing to AKT activation and subsequent FSTL1-induced survival and function of endothelial cells and cardiac myocytes (PubMed:20054002). {ECO:0000250|UniProtKB:Q8BWT5, ECO:0000269|PubMed:20054002}.

Molecular Weight: 170.4 kDa

UniProt: Q14689

Pathways: M Phase