LIG3 Protein (AA 43-1009) (His tag)

Catalog No. ABIN3092191

Product Details

Target: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Protein Type: Recombinant

Protein Characteristics: AA 43-1009

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This LIG3 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: LLHGHPLFLR RKPVLSFQGS HLRSRATYLV FLPGLHVGLC SGPCEMAEQR FCVDYAKRGT AGCKKCKEKI VKGVCRIGKV VPNPFSESGG DMKEWYHIKC MFEKLERARA TTKKIEDLTE LEGWEELEDN EKEQITQHIA DLSSKAAGTP KKKAVVQAKL TTTGQVTSPV KGASFVTSTN PRKFSGFSAK PNNSGEAPSS PTPKRSLSSS KCDPRHKDCL LREFRKLCAM VADNPSYNTK TQIIQDFLRK GSAGDGFHGD VYLTVKLLLP GVIKTVYNLN DKQIVKLFSR IFNCNPDDMA RDLEQGDVSE TIRVFFEQSK SFPPAAKSLL TIQEVDEFLL RLSKLTKEDE QQQALQDIAS RCTANDLKCI IRLIKHDLKM NSGAKHVLDA LDPNAYEAFK ASRNLQDVVE RVLHNAQEVE KEPGQRRALS VQASLMTPVQ PMLAEACKSV EYAMKKCPNG MFSEIKYDGE RVQVHKNGDH FSYFSRSLKP VLPHKVAHFK DYIPQAFPGG HSMILDSEVL LIDNKTGKPL PFGTLGVHKK AAFQDANVCL FVFDCIYFND VSLMDRPLCE RRKFLHDNMV EIPNRIMFSE MKRVTKALDL ADMITRVIQE GLEGLVLKDV KGTYEPGKRH WLKVKKDYLN EGAMADTADL VVLGAFYGQG SKGGMMSIFL MGCYDPGSQK WCTVTKCAGG HDDATLARLQ NELDMVKISK DPSKIPSWLK VNKIYYPDFI VPDPKKAAVW EITGAEFSKS EAHTADGISI RFPRCTRIRD DKDWKSATNL PQLKELYQLS KEKADFTVVA GDEGSSTTGG SSEENKGPSG SAVSRKAPSK PSASTKKAEG KLSNSNSKDG NMQTAKPSAM KVGEKLATKS SPVKVGEKRK AADETLCQTK VLLDIFTGVR LYLPPSTPDF SRLRRYFVAF DGDLVQEFDM TSATHVLGSR DKNPAAQQVS PEWIWACIRK RRLVAPC
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human LIG3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: LIG3 (Ligase III, DNA, ATP-Dependent (LIG3))

Alternative Name: LIG3 (LIG3 Products)

Synonyms: orf36 Protein, DDBDRAFT_0218523 Protein, DDBDRAFT_0232266 Protein, DDB_0218523 Protein, DDB_0232266 Protein, LIG3 Protein, wu:fb12c02 Protein, wu:fd10b03 Protein, wu:fm79b09 Protein, LIG2 Protein, D11Wsu78e Protein, An02g06890 Protein, AO090003000668 Protein, lig3 Protein, DNA ligase III Protein, BRCT domain-containing protein Protein, DNA ligase 3 Protein, ligase III, DNA, ATP-dependent Protein, ligase III, DNA, ATP-dependent L homeolog Protein, orf36 Protein, lig3 Protein, LIG3 Protein, Lig3 Protein, Bm1_22840 Protein, ANI_1_2644024 Protein, AOR_1_1174154 Protein, LOC100634887 Protein, lig3.L Protein

Background: Isoform 3 functions as heterodimer with DNA-repair protein XRCC1 in the nucleus and can correct defective DNA strand-break repair and sister chromatid exchange following treatment with ionizing radiation and alkylating agents. Isoform 1 is targeted to mitochondria, where it functions as DNA ligase in mitochondrial base-excision DNA repair (PubMed:10207110, PubMed:24674627). {ECO:0000269|PubMed:10207110, ECO:0000269|PubMed:24674627}.

Molecular Weight: 108.6 kDa Including tag.

UniProt: P49916

Pathways: DNA Damage Repair