E2F8 Protein (AA 1-867) (Strep Tag)

Catalog No. ABIN3092279

Product Details

Target: E2F8 (E2F Transcription Factor 8 (E2F8))

Protein Type: Recombinant

Protein Characteristics: AA 1-867

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This E2F8 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MENEKENLFC EPHKRGLMKT PLKESTTANI VLAEIQPDFG PLTTPTKPKE GSQGEPWTPT ANLKMLISAV SPEIRNRDQK RGLFDNRSGL PEAKDCIHEH LSGDEFEKSQ PSRKEKSLGL LCHKFLARYP NYPNPAVNND ICLDEVAEEL NVERRRIYDI VNVLESLHMV SRLAKNRYTW HGRHNLNKTL GTLKSIGEEN KYAEQIMMIK KKEYEQEFDF IKSYSIEDHI IKSNTGPNGH PDMCFVELPG VEFRAASVNS RKDKSLRVMS QKFVMLFLVS TPQIVSLEVA AKILIGEDHV EDLDKSKFKT KIRRLYDIAN VLSSLDLIKK VHVTEERGRK PAFKWTGPEI SPNTSGSSPV IHFTPSDLEV RRSSKENCAK NLFSTRGKPN FTRHPSLIKL VKSIESDRRK INSAPSSPIK TNKAESSQNS APFPSKMAQL AAICKMQLEE QSSESRQKVK VQLARSGPCK PVAPLDPPVN AEMELTAPSL IQPLGMVPLI PSPLSSAVPL ILPQAPSGPS YAIYLQPTQA HQSVTPPQGL SPTVCTTHSS KATGSKDSTD ATTEKAANDT SKASASTRPG SLLPAPERQG AKSRTREPAG ERGSKRASML EDSGSKKKFK EDLKGLENVS ATLFPSGYLI PLTQCSSLGA ESILSGKENS SALSPNHRIY SSPIAGVIPV TSSELTAVNF PSFHVTPLKL MVSPTSVAAV PVGNSPALAS SHPVPIQNPS SAIVNFTLQH LGLISPNVQL SASPGSGIVP VSPRIESVNV APENAGTQQG RATNYDSPVP GQSQPNGQSV AVTGAQQPVP VTPKGSQLVA ESFFRTPGGP TKPTSSSCMD FEGANKTSLG TLFVPQRKLE VSTEDVH
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: E2F8 (E2F Transcription Factor 8 (E2F8))

Alternative Name: E2F8 (E2F8 Products)

Synonyms: MGC130750 Protein, E2F8 Protein, E2F-8 Protein, RGD1308091 Protein, 4432406C08Rik Protein, AA410048 Protein, E2F transcription factor 8 Protein, E2F transcription factor 8 L homeolog Protein, E2F8 Protein, e2f8.L Protein, e2f8 Protein, E2f8 Protein

Background: Transcription factor E2F8 (E2F-8),FUNCTION: Atypical E2F transcription factor that participates in various processes such as angiogenesis and polyploidization of specialized cells. Mainly acts as a transcription repressor that binds DNA independently of DP proteins and specifically recognizes the E2 recognition site 5'-TTTC[CG]CGC-3'. Directly represses transcription of classical E2F transcription factors such as E2F1: component of a feedback loop in S phase by repressing the expression of E2F1, thereby preventing p53/TP53-dependent apoptosis. Plays a key role in polyploidization of cells in placenta and liver by regulating the endocycle, probably by repressing genes promoting cytokinesis and antagonizing action of classical E2F proteins (E2F1, E2F2 and/or E2F3). Required for placental development by promoting polyploidization of trophoblast giant cells. Acts as a promoter of sprouting angiogenesis, possibly by acting as a transcription activator: associates with HIF1A, recognizes and binds the VEGFA promoter, which is different from canonical E2 recognition site, and activates expression of the VEGFA gene. {ECO:0000269|PubMed:15897886, ECO:0000269|PubMed:16179649, ECO:0000269|PubMed:18202719, ECO:0000269|PubMed:22903062}.

Molecular Weight: 94.2 kDa

UniProt: A0AVK6