EEF2 Protein (AA 2-858) (His tag)

Catalog No. ABIN3092283

Product Details

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Protein Type: Recombinant

Protein Characteristics: AA 2-858

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This EEF2 protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)

Sequence: VNFTVDQIRA IMDKKANIRN MSVIAHVDHG KSTLTDSLVC KAGIIASARA GETRFTDTRK DEQERCITIK STAISLFYEL SENDLNFIKQ SKDGAGFLIN LIDSPGHVDF SSEVTAALRV TDGALVVVDC VSGVCVQTET VLRQAIAERI KPVLMMNKMD RALLELQLEP EELYQTFQRI VENVNVIIST YGEGESGPMG NIMIDPVLGT VGFGSGLHGW AFTLKQFAEM YVAKFAAKGE GQLGPAERAK KVEDMMKKLW GDRYFDPANG KFSKSATSPE GKKLPRTFCQ LILDPIFKVF DAIMNFKKEE TAKLIEKLDI KLDSEDKDKE GKPLLKAVMR RWLPAGDALL QMITIHLPSP VTAQKYRCEL LYEGPPDDEA AMGIKSCDPK GPLMMYISKM VPTSDKGRFY AFGRVFSGLV STGLKVRIMG PNYTPGKKED LYLKPIQRTI LMMGRYVEPI EDVPCGNIVG LVGVDQFLVK TGTITTFEHA HNMRVMKFSV SPVVRVAVEA KNPADLPKLV EGLKRLAKSD PMVQCIIEES GEHIIAGAGE LHLEICLKDL EEDHACIPIK KSDPVVSYRE TVSEESNVLC LSKSPNKHNR LYMKARPFPD GLAEDIDKGE VSARQELKQR ARYLAEKYEW DVAEARKIWC FGPDGTGPNI LTDITKGVQY LNEIKDSVVA GFQWATKEGA LCEENMRGVR FDVHDVTLHA DAIHRGGGQI IPTARRCLYA SVLTAQPRLM EPIYLVEIQC PEQVVGGIYG VLNRKRGHVF EESQVAGTPM FVVKAYLPVN ESFGFTADLR SNTGGQAFPQ CVFDHWQILP GDPFDNSSRP SQVVAETRKR KGLKEGIPAL DNFLDKL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human EEF2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: EEF2 (Eukaryotic Translation Elongation Factor 2 (EEF2))

Alternative Name: EEF2 (EEF2 Products)

Synonyms: EEF-2 Protein, EF-2 Protein, EF2 Protein, Ef-2 Protein, eef2 Protein, MGC76191 Protein, MGC79628 Protein, EEF2 Protein, eef2l Protein, fe49h02 Protein, wu:fe49h02 Protein, zgc:63584 Protein, ef2 Protein, zef2 Protein, CG2238 Protein, Dmel\CG2238 Protein, EF-2b Protein, EF2B Protein, EF2b Protein, Ef2 Protein, Ef2B Protein, Ef2b Protein, anon-EST:Liang-1.44 Protein, chr2L:21668915..21669164 Protein, clone 1.44 Protein, eEF2 Protein, eF2 Protein, ef2b Protein, eukaryotic translation elongation factor 2 Protein, eukaryotic translation elongation factor 2, gene 1 Protein, eukaryotic translation elongation factor 2b Protein, Elongation factor 2 Protein, EEF2 Protein, Eef2 Protein, eef2.1 Protein, POSPLDRAFT_118836 Protein, eef2b Protein, eef-2 Protein, eef2 Protein, EF2 Protein

Background: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post-translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.

Molecular Weight: 96.2 kDa Including tag.

UniProt: P13639

Pathways: AMPK Signaling