EHMT2 Protein (AA 2-1210) (His tag)

Catalog No. ABIN3092368

Product Details

Target: EHMT2 (Ankyrin Repeat-Containing Protein (EHMT2))

Protein Type: Recombinant

Protein Characteristics: AA 2-1210

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This EHMT2 protein is labelled with His tag.

Application: ELISA, SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys)

Sequence: AAAAGAAAAA AAEGEAPAEM GALLLEKETR GATERVHGSL GDTPRSEETL PKATPDSLEP AGPSSPASVT VTVGDEGADT PVGATPLIGD ESENLEGDGD LRGGRILLGH ATKSFPSSPS KGGSCPSRAK MSMTGAGKSP PSVQSLAMRL LSMPGAQGAA AAGSEPPPAT TSPEGQPKVH RARKTMSKPG NGQPPVPEKR PPEIQHFRMS DDVHSLGKVT SDLAKRRKLN SGGGLSEELG SARRSGEVTL TKGDPGSLEE WETVVGDDFS LYYDSYSVDE RVDSDSKSEV EALTEQLSEE EEEEEEEEEE EEEEEEEEEE EEDEESGNQS DRSGSSGRRK AKKKWRKDSP WVKPSRKRRK REPPRAKEPR GVNGVGSSGP SEYMEVPLGS LELPSEGTLS PNHAGVSNDT SSLETERGFE ELPLCSCRME APKIDRISER AGHKCMATES VDGELSGCNA AILKRETMRP SSRVALMVLC ETHRARMVKH HCCPGCGYFC TAGTFLECHP DFRVAHRFHK ACVSQLNGMV FCPHCGEDAS EAQEVTIPRG DGVTPPAGTA APAPPPLSQD VPGRADTSQP SARMRGHGEP RRPPCDPLAD TIDSSGPSLT LPNGGCLSAV GLPLGPGREA LEKALVIQES ERRKKLRFHP RQLYLSVKQG ELQKVILMLL DNLDPNFQSD QQSKRTPLHA AAQKGSVEIC HVLLQAGANI NAVDKQQRTP LMEAVVNNHL EVARYMVQRG GCVYSKEEDG STCLHHAAKI GNLEMVSLLL STGQVDVNAQ DSGGWTPIIW AAEHKHIEVI RMLLTRGADV TLTDNEENIC LHWASFTGSA AIAEVLLNAR CDLHAVNYHG DTPLHIAARE SYHDCVLLFL SRGANPELRN KEGDTAWDLT PERSDVWFAL QLNRKLRLGV GNRAIRTEKI ICRDVARGYE NVPIPCVNGV DGEPCPEDYK YISENCETST MNIDRNITHL QHCTCVDDCS SSNCLCGQLS IRCWYDKDGR LLQEFNKIEP PLIFECNQAC SCWRNCKNRV VQSGIKVRLQ LYRTAKMGWG VRALQTIPQG TFICEYVGEL ISDAEADVRE DDSYLFDLDN KDGEVYCIDA RYYGNISRFI NHLCDPNIIP VRVFMLHQDL RFPRIAFFSS RDIRTGEELG FDYGDRFWDI KSKYFTCQCG SEKCKHSAEA IALEQSRLAR LDPHPELLPE LGSLPPVNT
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human EHMT2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: EHMT2 (Ankyrin Repeat-Containing Protein (EHMT2))

Alternative Name: EHMT2 (EHMT2 Products)

Synonyms: BAT8 Protein, C6orf30 Protein, G9A Protein, GAT8 Protein, KMT1C Protein, NG36 Protein, T27C4.12 Protein, T27C4_12 Protein, DL3235W Protein, FCAALL.188 Protein, T18B16.120 Protein, T18B16_120 Protein, F9H3.13 Protein, F9H3_13 Protein, F9H3.7 Protein, F9H3_7 Protein, F9H3.9 Protein, F9H3_9 Protein, F9H3.6 Protein, F9H3_6 Protein, F13G24.40 Protein, T20K14.110 Protein, T20K14_110 Protein, MWD22.10 Protein, MWD22_10 Protein, MRB17.12 Protein, MRB17_12 Protein, MAF19.22 Protein, MAF19_22 Protein, Bat8 Protein, D17Ertd710e Protein, G9a Protein, Ng36 Protein, euchromatic histone lysine methyltransferase 2 Protein, ankyrin repeat-containing protein Protein, Ankyrin repeat family protein Protein, euchromatic histone lysine N-methyltransferase 2 Protein, EHMT2 Protein, ank1 Protein, IscW_ISCW006888 Protein, Arnit_2917 Protein, AT3G04470 Protein, AT4G14390 Protein, AT4G19150 Protein, AT4G03500 Protein, AT4G03450 Protein, AT4G03460 Protein, AT4G03440 Protein, PIA1 Protein, AT5G15500 Protein, AT5G51160 Protein, AT5G54620 Protein, ANK6 Protein, AT1G04780 Protein, AT1G62050 Protein, Ehmt2 Protein

Background: Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. May also methylate histone H1. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53. Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself. {ECO:0000269|PubMed:11316813, ECO:0000269|PubMed:18438403, ECO:0000269|PubMed:20084102, ECO:0000269|PubMed:20118233, ECO:0000269|PubMed:22387026, ECO:0000269|PubMed:8457211}.

Molecular Weight: 133.2 kDa Including tag.

UniProt: Q96KQ7