ELAVL1 Protein (AA 2-326) (His tag)

Catalog No. ABIN3092443

Product Details

Target: ELAVL1 (ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) (ELAVL1))

Protein Type: Recombinant

Protein Characteristics: AA 2-326

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This ELAVL1 protein is labelled with His tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)

Sequence: SNGYEDHMAE DCRGDIGRTN LIVNYLPQNM TQDELRSLFS SIGEVESAKL IRDKVAGHSL GYGFVNYVTA KDAERAINTL NGLRLQSKTI KVSYARPSSE VIKDANLYIS GLPRTMTQKD VEDMFSRFGR IINSRVLVDQ TTGLSRGVAF IRFDKRSEAE EAITSFNGHK PPGSSEPITV KFAANPNQNK NVALLSQLYH SPARRFGGPV HHQAQRFRFS PMGVDHMSGL SGVNVPGNAS SGWCIFIYNL GQDADEGILW QMFGPFGAVT NVKVIRDFNT NKCKGFGFVT MTNYEEAAMA IASLNGYRLG DKILQVSFKT NKSHK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human ELAVL1 Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ELAVL1 (ELAV (Embryonic Lethal, Abnormal Vision, Drosophila)-Like 1 (Hu Antigen R) (ELAVL1))

Alternative Name: ELAVL1 (ELAVL1 Products)

Synonyms: ELAV1 Protein, HUR Protein, Hua Protein, MelG Protein, HuR Protein, 2410055N02Rik Protein, W91709 Protein, elra Protein, fa25f08 Protein, fb59a02 Protein, fc60g02 Protein, hua Protein, wu:fa25f08 Protein, wu:fb59a02 Protein, wu:fc60g02 Protein, zHuA Protein, Elavl1 Protein, Xel-1 Protein, elav1 Protein, elrA Protein, hur Protein, melg Protein, p36 Protein, ElrA Protein, elavl1 Protein, elavl1-a Protein, elavl1-b Protein, ELAV like RNA binding protein 1 Protein, ELAV-like protein 1 Protein, ELAV (embryonic lethal, abnormal vision)-like 1 (Hu antigen R) Protein, ELAV like RNA binding protein 1a Protein, ELAV like RNA binding protein 1 L homeolog Protein, ELAV like RNA binding protein 1 S homeolog Protein, ELAVL1 Protein, Elavl1 Protein, elav1 Protein, elavl1a Protein, elavl1.L Protein, elavl1.S Protein

Background: RNA-binding protein that binds to the 3'-UTR region of mRNAs and increases their stability (PubMed:14517288, PubMed:18285462). Involved in embryonic stem cells (ESCs) differentiation: preferentially binds mRNAs that are not methylated by N6-methyladenosine (m6A), stabilizing them, promoting ESCs differentiation (By similarity). Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed:8626503, PubMed:17632515, PubMed:18285462, PubMed:23519412). Binds avidly to the AU-rich element in FOS and IL3/interleukin-3 mRNAs. In the case of the FOS AU-rich element, binds to a core element of 27 nucleotides that contain AUUUA, AUUUUA, and AUUUUUA motifs. Binds preferentially to the 5'-UUUU[AG]UUU-3' motif in vitro (PubMed:8626503). With ZNF385A, binds the 3'-UTR of p53/TP53 mRNA to control their nuclear export induced by CDKN2A. Hence, may regulate p53/TP53 expression and mediate in part the CDKN2A anti-proliferative activity. May also bind with ZNF385A the CCNB1 mRNA (By similarity). {ECO:0000250|UniProtKB:P70372, ECO:0000269|PubMed:14517288, ECO:0000269|PubMed:17632515, ECO:0000269|PubMed:18285462, ECO:0000269|PubMed:19029303, ECO:0000269|PubMed:23519412, ECO:0000269|PubMed:8626503}.

Molecular Weight: 36.9 kDa Including tag.

UniProt: Q15717

Pathways: AMPK Signaling