EPH Receptor A5 Protein (EPHA5) (AA 25-1037) (rho-1D4 tag)

Catalog No. ABIN3092452

Product Details

Target: EPH Receptor A5 (EPHA5)

Protein Type: Recombinant

Protein Characteristics: AA 25-1037

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This EPH Receptor A5 protein is labelled with rho-1D4 tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: PASLAGCYSA PRRAPLWTCL LLCAALRTLL ASPSNEVNLL DSRTVMGDLG WIAFPKNGWE EIGEVDENYA PIHTYQVCKV MEQNQNNWLL TSWISNEGAS RIFIELKFTL RDCNSLPGGL GTCKETFNMY YFESDDQNGR NIKENQYIKI DTIAADESFT ELDLGDRVMK LNTEVRDVGP LSKKGFYLAF QDVGACIALV SVRVYYKKCP SVVRHLAVFP DTITGADSSQ LLEVSGSCVN HSVTDEPPKM HCSAEGEWLV PIGKCMCKAG YEEKNGTCQV CRPGFFKASP HIQSCGKCPP HSYTHEEAST SCVCEKDYFR RESDPPTMAC TRPPSAPRNA ISNVNETSVF LEWIPPADTG GRKDVSYYIA CKKCNSHAGV CEECGGHVRY LPRQSGLKNT SVMMVDLLAH TNYTFEIEAV NGVSDLSPGA RQYVSVNVTT NQAAPSPVTN VKKGKIAKNS ISLSWQEPDR PNGIILEYEI KYFEKDQETS YTIIKSKETT ITAEGLKPAS VYVFQIRART AAGYGVFSRR FEFETTPVFA ASSDQSQIPV IAVSVTVGVI LLAVVIGVLL SGSCCECGCG RASSLCAVAH PSLIWRCGYS KAKQDPEEEK MHFHNGHIKL PGVRTYIDPH TYEDPNQAVH EFAKEIEASC ITIERVIGAG EFGEVCSGRL KLPGKRELPV AIKTLKVGYT EKQRRDFLGE ASIMGQFDHP NIIHLEGVVT KSKPVMIVTE YMENGSLDTF LKKNDGQFTV IQLVGMLRGI SAGMKYLSDM GYVHRDLAAR NILINSNLVC KVSDFGLSRV LEDDPEAAYT TRGGKIPIRW TAPEAIAFRK FTSASDVWSY GIVMWEVVSY GERPYWEMTN QDVIKAVEEG YRLPSPMDCP AALYQLMLDC WQKERNSRPK FDEIVNMLDK LIRNPSSLKT LVNASCRVSN LLAEHSPLGS GAYRSVGEWL EAIKMGRYTE IFMENGYSSM DAVAQVTLED LRRLGVTLVG HQKKIMNSLQ EMKVQLVNGM VPL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human EPHA5 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: EPH Receptor A5 (EPHA5)

Alternative Name: EPHA5 (EPHA5 Products)

Synonyms: CEK7 Protein, EHK-1 Protein, EHK1 Protein, EK7 Protein, HEK7 Protein, TYRO4 Protein, AI854630 Protein, AW125296 Protein, Cek7 Protein, Ehk1 Protein, Els1 Protein, Hek7 Protein, Rek7 Protein, bsk Protein, Els1. Protein, EPHA5 Protein, EPH receptor A5 Protein, Eph receptor A5 Protein, EPHA5 Protein, epha5 Protein, Epha5 Protein

Background: Receptor tyrosine kinase which binds promiscuously GPI-anchored ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Among GPI-anchored ephrin-A ligands, EFNA5 most probably constitutes the cognate/functional ligand for EPHA5. Functions as an axon guidance molecule during development and may be involved in the development of the retinotectal, entorhino-hippocampal and hippocamposeptal pathways. Together with EFNA5 plays also a role in synaptic plasticity in adult brain through regulation of synaptogenesis. In addition to its function in the nervous system, the interaction of EPHA5 with EFNA5 mediates communication between pancreatic islet cells to regulate glucose-stimulated insulin secretion (By similarity). {ECO:0000250}.

Molecular Weight: 113.6 kDa Including tag.

UniProt: P54756

Pathways: RTK Signaling