EXOC7 Protein (AA 1-735) (Strep Tag)

Catalog No. ABIN3092467

Product Details

Target: EXOC7 (Exocyst Complex Component 7 (EXOC7))

Protein Type: Recombinant

Protein Characteristics: AA 1-735

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This EXOC7 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MIPPQEASAR RREIEDKLKQ EEETLSFIRD SLEKSDQLTK NMVSILSSFE SRLMKLENSI IPVHKQTENL QRLQENVEKT LSCLDHVISY YHVASDTEKI IREGPTGRLE EYLGSMAKIQ KAVEYFQDNS PDSPELNKVK LLFERGKEAL ESEFRSLMTR HSKVVSPVLI LDLISGDDDL EAQEDVTLEH LPESVLQDVI RISRWLVEYG RNQDFMNVYY QIRSSQLDRS IKGLKEHFHK SSSSSGVPYS PAIPNKRKDT PTKKPVKRPG TIRKAQNLLK QYSQHGLDGK KGGSNLIPLE GLLPCTPRGG LPGPWINAAC VCAADISPGH EHDFRVKHLS EALNDKHGPL AGRDDMLDVE TDAYIHCVSA FVKLAQSEYQ LLADIIPEHH QKKTFDSLIQ DALDGLMLEG ENIVSAARKA IVRHDFSTVL TVFPILRHLK QTKPEFDQVL QGTAASTKNK LPGLITSMET IGAKALEDFA DNIKNDPDKE YNMPKDGTVH ELTSNAILFL QQLLDFQETA GAMLASQETS SSATSYSSEF SKRLLSTYIC KVLGNLQLNL LSKSKVYEDP ALSAIFLHNN YNYILKSLEK SELIQLVAVT QKTAERSYRE HIEQQIQTYQ RSWLKVTDYI AEKNLPVFQP GVKLRDKERQ IIKERFKGFN DGLEELCKIQ KAWAIPDTEQ RDRIRQAQKT IVKETYGAFL QKFGSVPFTK NPEKYIKYGV EQVGDMIDRL FDTSA
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: EXOC7 (Exocyst Complex Component 7 (EXOC7))

Alternative Name: EXOC7 (EXOC7 Products)

Synonyms: 2-5-3p Protein, EX070 Protein, EXO70 Protein, EXOC1 Protein, Exo70p Protein, YJL085W Protein, Exo70 Protein, sec70 Protein, zgc:56575 Protein, exoc7 Protein, EXOC7 Protein, DDBDRAFT_0217074 Protein, DDBDRAFT_0233927 Protein, DDB_0217074 Protein, DDB_0233927 Protein, DKFZp459C0924 Protein, exocyst complex component 7 Protein, exocyst complex subunit 7 Protein, exocyst complex component 7 L homeolog Protein, EXOC7 Protein, Exoc7 Protein, exoc7 Protein, exoc7.L Protein

Background: Exocyst complex component 7 (Exocyst complex component Exo70),FUNCTION: Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. In adipocytes, plays a crucial role in targeting SLC2A4 vesicle to the plasma membrane in response to insulin, perhaps directing the vesicle to the precise site of fusion (By similarity). It is required for neuron survival and plays an essential role in cortical development (By similarity). {ECO:0000250, ECO:0000250|UniProtKB:E7FC72}.

Molecular Weight: 83.4 kDa

UniProt: Q9UPT5

Pathways: Peptide Hormone Metabolism, ER-Nucleus Signaling, Synaptic Vesicle Exocytosis