FMN1 Protein (AA 1-1419) (Strep Tag)

Catalog No. ABIN3092631

Product Details

Target: FMN1 (Formin 1 (FMN1))

Protein Type: Recombinant

Protein Characteristics: AA 1-1419

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This FMN1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MEGTHCTLQL HKPITELCYI SFCLPKGEVR GFSYKGTVTL DRSNKGFHNC YQVREESDII SLSQEPDEHP GDIFFKQTPT KDILTELYKL TTERERLLTN LLSSDHILGI TMGNQEGKLQ ELSVSLAPED DCFQSAGDWQ GELPVGPLNK RSTHGNKKPR RSSGRRESFG ALPQKRTKRK GRGGRESAPL MGKDKICSSH SLPLSRTRPN LWVLEEKGNL LPNGALACSL QRRESCPPDI PKTPDTDLGF GSFETAFKDT GLGREVLPPD CSSTEAGGDG IRRPPSGLEH QQTGLSESHQ DPEKHPEAEK DEMEKPAKRT CKQKPVSKVV AKVQDLSSQV QRVVKTHSKG KETIAIRPAA HAEFVPKADL LTLPGAEAGA HGSRRQGKER QGDRSSQSPA GETASISSVS ASAEGAVNKV PLKVIESEKL DEAPEGKRLG FPVHTSVPHT RPETRNKRRA GLPLGGHKSL FLDLPHKVGP DSSQPRGDKK KPSPPAPAAL GKVFNNSASQ SSTHKQTSPV PSPLSPRLPS PQQHHRILRL PALPGEREAA LNDSPCRKSR VFSGCVSADT LEPPSSAKVT ETKGASPAFL RAGQPRLVPG ETLEKSLGPG KTTAEPQHQS PPGISSEGFP WDGFNEQTPK DLPNRDGGAW VLGYRAGPAC PFLLHEEREK SNRSELYLDL HPDHSLTEQD DRTPGRLQAV WPPPKTKDTE EKVGLKYTEA EYQAAILHLK REHKEEIENL QAQFELRAFH IRGEHAMITA RLEETIENLK HELEHRWRGG CEERKDVCIS TDDDCPPKTF RNVCVQTDRE TFLKPCESES KTTRSNQLVP KKLNISSLSQ LSPPNDHKDI HAALQPMEGM ASNQQKALPP PPASIPPPPP LPSGLGSLSP APPMPPVSAG PPLPPPPPPP PPLPPPSSAG PPPPPPPPPL PNSPAPPNPG GPPPAPPPPG LAPPPPPGLF FGLGSSSSQC PRKPAIEPSC PMKPLYWTRI QISDRSQNAT PTLWDSLEEP DIRDPSEFEY LFSKDTTQQK KKPLSETYEK KNKVKKIIKL LDGKRSQTVG ILISSLHLEM KDIQQAIFNV DDSVVDLETL AALYENRAQE DELVKIRKYY ETSKEEELKL LDKPEQFLHE LAQIPNFAER AQCIIFRSVF SEGITSLHRK VEIITRASKD LLHVKSVKDI LALILAFGNY MNGGNRTRGQ ADGYSLEILP KLKDVKSRDN GINLVDYVVK YYLRYYDQEA GTEKSVFPLP EPQDFFLASQ VKFEDLIKDL RKLKRQLEAS EKQMVVVCKE SPKEYLQPFK DKLEEFFQKA KKEHKMEESH LENAQKSFET TVRYFGMKPK SGEKEITPSY VFMVWYEFCS DFKTIWKRES KNISKERLKM AQESVSKLTS EKKVETKKIN PTASLKERLR QKEASVTTN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: FMN1 (Formin 1 (FMN1))

Alternative Name: FMN1 (FMN1 Products)

Synonyms: si:dkey-81b15.10 Protein, fmn Protein, formin Protein, FMN Protein, LD Protein, BB164513 Protein, Fmn Protein, formin-1 Protein, ld Protein, RGD1306349 Protein, RGD1310100 Protein, formin 1 Protein, fmn1 Protein, FMN1 Protein, Fmn1 Protein

Background: Formin-1 (Limb deformity protein homolog),FUNCTION: Plays a role in the formation of adherens junction and the polymerization of linear actin cables. {ECO:0000250}.

Molecular Weight: 157.6 kDa

UniProt: Q68DA7

Pathways: Regulation of Actin Filament Polymerization