GLDC Protein (AA 36-1020) (His tag)

Catalog No. ABIN3092722

Product Details

Target: GLDC (Glycine Dehydrogenase (GLDC))

Protein Type: Recombinant

Protein Characteristics: AA 36-1020

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This GLDC protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: DSSSGGGDSA AAGASRLLER LLPRHDDFAR RHIGPGDKDQ REMLQTLGLA SIDELIEKTV PANIRLKRPL KMEDPVCENE ILATLHAISS KNQIWRSYIG MGYYNCSVPQ TILRNLLENS GWITQYTPYQ PEVSQGRLES LLNYQTMVCD ITGLDMANAS LLDEGTAAAE ALQLCYRHNK RRKFLVDPRC HPQTIAVVQT RAKYTGVLTE LKLPCEMDFS GKDVSGVLFQ YPDTEGKVED FTELVERAHQ SGSLACCATD LLALCILRPP GEFGVDIALG SSQRFGVPLG YGGPHAAFFA VRESLVRMMP GRMVGVTRDA TGKEVYRLAL QTREQHIRRD KATSNICTAQ ALLANMAAMF AIYHGSHGLE HIARRVHNAT LILSEGLKRA GHQLQHDLFF DTLKIQCGCS VKEVLGRAAQ RQINFRLFED GTLGISLDET VNEKDLDDLL WIFGCESSAE LVAESMGEEC RGIPGSVFKR TSPFLTHQVF NSYHSETNIV RYMKKLENKD ISLVHSMIPL GSCTMKLNSS SELAPITWKE FANIHPFVPL DQAQGYQQLF RELEKDLCEL TGYDQVCFQP NSGAQGEYAG LATIRAYLNQ KGEGHRTVCL IPKSAHGTNP ASAHMAGMKI QPVEVDKYGN IDAVHLKAMV DKHKENLAAI MITYPSTNGV FEENISDVCD LIHQHGGQVY LDGANMNAQV GICRPGDFGS DVSHLNLHKT FCIPHGGGGP GMGPIGVKKH LAPFLPNHPV ISLKRNEDAC PVGTVSAAPW GSSSILPISW AYIKMMGGKG LKQATETAIL NANYMAKRLE THYRILFRGA RGYVGHEFIL DTRPFKKSAN IEAVDVAKRL QDYGFHAPTM SWPVAGTLMV EPTESEDKAE LDRFCDAMIS IRQEIADIEE GRIDPRVNPL KMSPHSLTCV TSSHWDRPYS REVAAFPLPF VKPENKFWPT IARIDDIYGD QHLVCTCPPM EVYESPFSEQ KRASS
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human GLDC Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: GLDC (Glycine Dehydrogenase (GLDC))

Alternative Name: GLDC (GLDC Products)

Synonyms: PSPTO1276 Protein, Tb07.43M14.350 Protein, DDBDRAFT_0219205 Protein, DDBDRAFT_0231130 Protein, DDB_0219205 Protein, DDB_0231130 Protein, D030049L12Rik Protein, D19Wsu57e Protein, GCE Protein, GCSP Protein, HYGN1 Protein, fb23b05 Protein, wu:fb23b05 Protein, zgc:66198 Protein, gldc Protein, glycine dehydrogenase (decarboxylating) GcvP Protein, glycine dehydrogenase Protein, glycine cleavage system P-protein Protein, glycine decarboxylase Protein, glycine dehydrogenase (decarboxylating) Protein, glycine decarboxylase S homeolog Protein, gcvP Protein, Tb927.7.1910 Protein, Gldc Protein, GLDC Protein, gldc Protein, gldc.S Protein

Background: The glycine cleavage system catalyzes the degradation of glycine. The P protein (GLDC) binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor, CO(2) is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein (GCSH).

Molecular Weight: 110.1 kDa Including tag.

UniProt: P23378