IGSF8 Protein (AA 28-613) (rho-1D4 tag)

Catalog No. ABIN3093021

Product Details

Target: IGSF8 (Immunoglobulin Superfamily, Member 8 (IGSF8))

Protein Type: Recombinant

Protein Characteristics: AA 28-613

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This IGSF8 protein is labelled with rho-1D4 tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: REVLVPEGPL YRVAGTAVSI SCNVTGYEGP AQQNFEWFLY RPEAPDTALG IVSTKDTQFS YAVFKSRVVA GEVQVQRLQG DAVVLKIARL QAQDAGIYEC HTPSTDTRYL GSYSGKVELR VLPDVLQVSA APPGPRGRQA PTSPPRMTVH EGQELALGCL ARTSTQKHTH LAVSFGRSVP EAPVGRSTLQ EVVGIRSDLA VEAGAPYAER LAAGELRLGK EGTDRYRMVV GGAQAGDAGT YHCTAAEWIQ DPDGSWAQIA EKRAVLAHVD VQTLSSQLAV TVGPGERRIG PGEPLELLCN VSGALPPAGR HAAYSVGWEM APAGAPGPGR LVAQLDTEGV GSLGPGYEGR HIAMEKVASR TYRLRLEAAR PGDAGTYRCL AKAYVRGSGT RLREAASARS RPLPVHVREE GVVLEAVAWL AGGTVYRGET ASLLCNISVR GGPPGLRLAA SWWVERPEDG ELSSVPAQLV GGVGQDGVAE LGVRPGGGPV SVELVGPRSH RLRLHSLGPE DEGVYHCAPS AWVQHADYSW YQAGSARSGP VTVYPYMHAL DTLFVPLLVG TGVALVTGAT VLGTITCCFM KRLRKR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human IGSF8 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:

1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin-free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: IGSF8 (Immunoglobulin Superfamily, Member 8 (IGSF8))

Alternative Name: IGSF8 (IGSF8 Products)

Synonyms: MGC77222 Protein, IGSF8 Protein, MGC151661 Protein, CD316 Protein, CD81P3 Protein, EWI-2 Protein, EWI2 Protein, KCT-4 Protein, LIR-D1 Protein, PGRL Protein, AA033172 Protein, AU041109 Protein, ESTM34 Protein, R75384 Protein, immunoglobulin superfamily, member 8 Protein, immunoglobulin superfamily member 8 Protein, igsf8 Protein, IGSF8 Protein, Igsf8 Protein

Background: May play a key role in diverse functions ascribed to CD81 and CD9 such as oocytes fertilization or hepatitis C virus function. May regulate proliferation and differentiation of keratinocytes. May be a negative regulator of cell motility: suppresses T-cell mobility coordinately with CD81, associates with CD82 to suppress prostate cancer cell migration, regulates epidermoid cell reaggregation and motility on laminin-5 with CD9 and CD81 as key linkers. May also play a role on integrin-dependent morphology and motility functions. May participate in the regulation of neurite outgrowth and maintenance of the neural network in the adult brain. {ECO:0000269|PubMed:11504738, ECO:0000269|PubMed:12750295, ECO:0000269|PubMed:12752121, ECO:0000269|PubMed:14662754, ECO:0000269|PubMed:15070678}.

Molecular Weight: 63.3 kDa Including tag.

UniProt: Q969P0