KCNMA1 Protein (AA 389-1236) (His tag)

Catalog No. ABIN3093393

Product Details

Target: KCNMA1 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1))

Protein Type: Recombinant

Protein Characteristics: AA 389-1236

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This KCNMA1 protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: ELIGNRKKYG GSYSAVSGRK HIVVCGHITL ESVSNFLKDF LHKDRDDVNV EIVFLHNISP NLELEALFKR HFTQVEFYQG SVLNPHDLAR VKIESADACL ILANKYCADP DAEDASNIMR VISIKNYHPK IRIITQMLQY HNKAHLLNIP SWNWKEGDDA ICLAELKLGF IAQSCLAQGL STMLANLFSM RSFIKIEEDT WQKYYLEGVS NEMYTEYLSS AFVGLSFPTV CELCFVKLKL LMIAIEYKSA NRESRILINP GNHLKIQEGT LGFFIASDAK EVKRAFFYCK ACHDDITDPK RIKKCGCKRP KMSIYKRMRR ACCFDCGRSE RDCSCMSGRV RGNVDTLERA FPLSSVSVND CSTSFRAFED EQPSTLSPKK KQRNGGMRNS PNTSPKLMRH DPLLIPGNDQ IDNMDSNVKK YDSTGMFHWC APKEIEKVIL TRSEAAMTVL SGHVVVCIFG DVSSALIGLR NLVMPLRASN FHYHELKHIV FVGSIEYLKR EWETLHNFPK VSILPGTPLS RADLRAVNIN LCDMCVILSA NQNNIDDTSL QDKECILASL NIKSMQFDDS IGVLQANSQG FTPPGMDRSS PDNSPVHGML RQPSITTGVN IPIITELVND TNVQFLDQDD DDDPDTELYL TQPFACGTAF AVSVLDSLMS ATYFNDNILT LIRTLVTGGA TPELEALIAE ENALRGGYST PQTLANRDRC RVAQLALLDG PFADLGDGGC YGDLFCKALK TYNMLCFGIY RLRDAHLSTP SQCTKRYVIT NPPYEFELVP TDLIFCLMQF DHNAGQSRAS LSHSSHSSQS SSKKSSSVHS IPSTANRQNR PKSRESRDKQ KYVQEERL
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human KCNMA1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: KCNMA1 (Potassium Large Conductance Calcium-Activated Channel, Subfamily M, alpha Member 1 (KCNMA1))

Alternative Name: KCNMA1 (KCNMA1 Products)

Synonyms: BKTM Protein, KCa1.1 Protein, MaxiK Protein, SAKCA Protein, SLO Protein, SLO-ALPHA Protein, SLO1 Protein, bA205K10.1 Protein, mSLO1 Protein, DDBDRAFT_0190653 Protein, DDBDRAFT_0235401 Protein, DDB_0190653 Protein, DDB_0235401 Protein, KCNMA Protein, KCNMA1 Protein, 5730414M22Rik Protein, BKCa Protein, Slo Protein, Slo1 Protein, mSlo Protein, mSlo1 Protein, KCNMA1b Protein, KCNMA1c Protein, Kcnma Protein, bktm Protein, kcnma1-A Protein, sakca Protein, cSlo Protein, slo1 Protein, kcnma1 Protein, si:ch211-39f22.2 Protein, BcDNA:GH10751 Protein, CG10693 Protein, Dmel\CG10693 Protein, Dslo Protein, dSlo Protein, dSlo1 Protein, dslo Protein, fSlo Protein, potassium calcium-activated channel subfamily M alpha 1 Protein, calcium-activated BK potassium channel, alpha subunit Protein, potassium large conductance calcium-activated channel, subfamily M, alpha member 1 Protein, potassium calcium-activated channel subfamily M alpha 1 L homeolog Protein, potassium large conductance calcium-activated channel, subfamily M, alpha member 1a Protein, calcium-activated potassium channel subunit alpha-1 Protein, slowpoke Protein, KCNMA1 Protein, kcnma1 Protein, Kcnma1 Protein, kcnma1.L Protein, kcnma1a Protein, LOC100454095 Protein, slo Protein

Background: Potassium channel activated by both membrane depolarization or increase in cytosolic Ca(2+) that mediates export of K(+). It is also activated by the concentration of cytosolic Mg(2+). Its activation dampens the excitatory events that elevate the cytosolic Ca(2+) concentration and/or depolarize the cell membrane. It therefore contributes to repolarization of the membrane potential. Plays a key role in controlling excitability in a number of systems, such as regulation of the contraction of smooth muscle, the tuning of hair cells in the cochlea, regulation of transmitter release, and innate immunity. In smooth muscles, its activation by high level of Ca(2+), caused by ryanodine receptors in the sarcoplasmic reticulum, regulates the membrane potential. In cochlea cells, its number and kinetic properties partly determine the characteristic frequency of each hair cell and thereby helps to establish a tonotopic map. Kinetics of KCNMA1 channels are determined by alternative splicing, phosphorylation status and its combination with modulating beta subunits. Highly sensitive to both iberiotoxin (IbTx) and charybdotoxin (CTX).

Molecular Weight: 96.1 kDa Including tag.

UniProt: Q12791

Pathways: Regulation of Hormone Metabolic Process, Sensory Perception of Sound