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LNPEP Protein (AA 132-1025) (His tag)

Crystallography grade LNPEP Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3093476
  • Target See all LNPEP Proteins
    LNPEP (Leucyl/cystinyl Aminopeptidase (LNPEP))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 132-1025
    Origin
    • 6
    • 3
    Human
    Source
    • 4
    • 3
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This LNPEP protein is labelled with His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    MKTIIALSYI FCLVFA PRCTFTKEGC HKKNQSIGLI QPFATNGKLF PWAQIRLPTA VVPLRYELSL HPNLTSMTFR GSVTISVQAL QVTWNIILHS TGHNISRVTF MSAVSSQEKQ AEILEYAYHG QIAIVAPEAL LAGHNYTLKI EYSANISSSY YGFYGFSYTD ESNEKKYFAA TQFEPLAARS AFPCFDEPAF KATFIIKIIR DEQYTALSNM PKKSSVVLDD GLVQDEFSES VKMSTYLVAF IVGEMKNLSQ DVNGTLVSIY AVPEKIGQVH YALETTVKLL EFFQNYFEIQ YPLKKLDLVA IPDFEAGAME NWGLLTFREE TLLYDSNTSS MADRKLVTKI IAHELAHQWF GNLVTMKWWN DLWLNEGFAT FMEYFSLEKI FKELSSYEDF LDARFKTMKK DSLNSSHPIS SSVQSSEQIE EMFDSLSYFK GSSLLLMLKT YLSEDVFQHA VVLYLHNHSY ASIQSDDLWD SFNEVTNQTL DVKRMMKTWT LQKGFPLVTV QKKGKELFIQ QERFFLNMKP EIQPSDTSYL WHIPLSYVTE GRNYSKYQSV SLLDKKSGVI NLTEEVLWVK VNINMNGYYI VHYADDDWEA LIHQLKINPY VLSDKDRANL INNIFELAGL GKVPLKRAFD LINYLGNENH TAPITEALFQ TDLIYNLLEK LGYMDLASRL VTRVFKLLQN QIQQQTWTDE GTPSMRELRS ALLEFACTHN LGNCSTTAMK LFDDWMASNG TQSLPTDVMT TVFKVGAKTD KGWSFLLGKY ISIGSEAEKN KILEALASSE DVRKLYWLMK SSLNGDNFRT QKLSFIIRTV GRHFPGHLLA WDFVKENWNK LVQKFPLGSY TIQNIVAGST YLFSTKTHLS EVQAFFENQS EATFRLRCVQ EALEVIQLNI QWMEKNLKSL TWWLHHHHHH
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human LNPEP Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    The protein is purified from the cleared cell lysate using His-tag capture materials. Eluate fractions are analyzed by SDS-PAGE. Protein containing fractions are subjected to a second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    30 mM Hepes, pH 7.4, 2,5 mM EDTA, 100 mM NaCl
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    LNPEP (Leucyl/cystinyl Aminopeptidase (LNPEP))
    Alternative Name
    LNPEP (LNPEP Products)
    Synonyms
    fb73h12 Protein, wu:fb73h12 Protein, zgc:66103 Protein, 2010309L07Rik Protein, 4732490P18Rik Protein, CAP Protein, IRAP Protein, PLAP Protein, gp160 Protein, vp165 Protein, P-LAP Protein, Vp165 Protein, LNPEP Protein, leucyl and cystinyl aminopeptidase Protein, leucyl/cystinyl aminopeptidase L homeolog Protein, leucyl/cystinyl aminopeptidase Protein, LNPEP Protein, lnpep.L Protein, lnpep Protein, Lnpep Protein
    Background
    Release of an N-terminal amino acid, cleaves before cysteine, leucine as well as other amino acids. Degrades peptide hormones such as oxytocin, vasopressin and angiotensin III, and plays a role in maintaining homeostasis during pregnancy. May be involved in the inactivation of neuronal peptides in the brain. Cleaves Met-enkephalin and dynorphin. Binds angiotensin IV and may be the angiotensin IV receptor in the brain. {ECO:0000269|PubMed:11389728, ECO:0000269|PubMed:11707427, ECO:0000269|PubMed:1731608}.
    Molecular Weight
    103.6 kDa Including tag.
    UniProt
    Q9UIQ6
    Pathways
    Regulation of long-term Neuronal Synaptic Plasticity
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