CAZIP Protein (AA 1-1369) (His tag)
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- Target See all CAZIP (MTUS2) Proteins
- CAZIP (MTUS2) (Microtubule-associated Tumor Suppressor Candidate 2 (MTUS2))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-1369
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Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This CAZIP protein is labelled with His tag.
- Application
- ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
- Sequence
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MSVPVAPKKS CYTQLRDNRN AARNNNESIL SLGDTNANQI MLEVSSSHDE SKTCDLGDEI GNTNSSEPEN RTHFHKEFHQ LQGFGKGSQA GSASLKDFRL SSTIQRELNE EHTVERGTDS LQTTRSIQGP SLSSWRNVMS EASLDVLAKR DAEIPRHVPK DKLAKTLDNE ELRRHSLERA SSSVAAVGSL TPQHPQPLSL DSREARGQIP GGGEGPQKTL PDHAVPAAFP ATDSTSEGKS VRHPKPSTSE SKQSTPSETQ TVGAHVLQVC SEHTSHSAHP EPALNLTLAS KEIPSKLEAQ LGQGKGEAKL DLKYVPPRRV EQEGKAAQEG YLGCHKEENL SALEGRDPCG EAHPEATDAL GHLLNSDLHH LGVGRGNCEE KRGVNPGEQD SLHTTPKQGS ASLGGADNQP TGKISPCAGE KLGERTSSSF SPGDSHVAFI PNNLTDSKPL DVIEEERRLG SGNKDSVMVL VFNPSVGENK TEVPEPLDPQ SGRSEARESK EVTTSVAENR NLLENADKIE STSARADSVL NIPAPLHPET TVNMTYQPTT PSSSFQDVSV FGMDAGSPLV VPPPTDSARL LNTSPKVPDK NTCPSGIPKP VFTHSKDTPS SQEGMENYQV EKTEERTETK PIIMPKPKHV RPKIITYIRR NPQALGQVDA SLVPVGLPYA PPTCTMPLPH EEKAAGGDLK PSANLYEKFK PDLQKPRVFS SGLMVSGIKP PGHPFSQMSE KFLQEVTDHP GKEEFCSPPY AHYEVPPTFY RSAMLLKPQL GLGAMSRLPS AKSRILIASQ RSSASAIHPP GPITTATSLY SSDPSADLKK ASSSNAAKSN LPKSGLRPPG YSRLPAAKLA AFGFVRSSSV SSVSSTQSGD SAQPEQGRPA TRSTFGNEEQ PVLKASLPSK DTPKGAGRVA PPASSSVTAP RRSLLPAPKS TSTPAGTKKD AQKDQDTNKP AVSSPKRVAA STTKLHSPGY PKQRTAAARN GFPPKPDPQA REAERQLVLR LKERCEQQTR QLGVAQGELK RAICGFDALA VATQHFFRKN ESALVKEKEL SIELANIRDE VAFHTAKCEK LQKEKEELER RFEDEVKRLG WQQQAELQEL EERLQLQFEA EMARLQEEHG DQLLSIRCQH QEQVEDLTAS HDAALLEMEN NHTVAITILQ DDHDHKVQEL MSTHELEKKE LEENFEKLRL SLQDQVDTLT FQSQSLRDRA RRFEEALRKN TEEQLEIALA PYQHLEEDMK SLKQVLEMKN QQIHEQEKKI LELEKLAEKN IILEEKIQVL QQQNEDLKAR IDQNTVVTRQ LSEENANLQE YVEKETQEKK RLSRTNEELL WKLQTGDPTS PIKLSPTSPV YRGSSSGPSS PARVSTTPR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human MTUS2 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
- Top Product
- Discover our top product MTUS2 Protein
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- CAZIP (MTUS2) (Microtubule-associated Tumor Suppressor Candidate 2 (MTUS2))
- Alternative Name
- MTUS2 (MTUS2 Products)
- Synonyms
- KIAA0774 Protein, DKFZp468L0812 Protein, CAZIP Protein, ICIS Protein, TIP150 Protein, 5730592G18Rik Protein, A730013O20Rik Protein, AU067634 Protein, C130038G02Rik Protein, Cazip Protein, Gm763 Protein, mKIAA0774 Protein, microtubule associated tumor suppressor candidate 2 Protein, microtubule associated scaffold protein 2 Protein, MTUS2 Protein, Mtus2 Protein
- Background
- Binds microtubules. Together with MAPRE1 may target the microtubule depolymerase KIF2C to the plus-end of microtubules. May regulate the dynamics of microtubules at their growing distal tip. {ECO:0000269|PubMed:19543227}.
- Molecular Weight
- 151.1 kDa Including tag.
- UniProt
- Q5JR59
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