Nischarin Protein (NISCH) (AA 2-1504) (His tag)

Catalog No. ABIN3094087

Product Details

Target: Nischarin (NISCH)

Protein Type: Recombinant

Protein Characteristics: AA 2-1504

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This Nischarin protein is labelled with His tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)

Sequence: ATARTFGPER EAEPAKEARV VGSELVDTYT VYIIQVTDGS HEWTVKHRYS DFHDLHEKLV AERKIDKNLL PPKKIIGKNS RSLVEKREKD LEVYLQKLLA AFPGVTPRVL AHFLHFHFYE INGITAALAE ELFEKGEQLL GAGEVFAIGP LQLYAVTEQL QQGKPTCASG DAKTDLGHIL DFTCRLKYLK VSGTEGPFGT SNIQEQLLPF DLSIFKSLHQ VEISHCDAKH IRGLVASKPT LATLSVRFSA TSMKEVLVPE ASEFDEWEPE GTTLEGPVTA VIPTWQALTT LDLSHNSVSE IDESVKLIPK IEFLDLSHNG LLVVDNLQHL YNLVHLDLSY NKLSSLEGLH TKLGNIKTLN LAGNLLESLS GLHKLYSLVN LDLRDNRIEQ MEEVRSIGSL PCLEHVSLLN NPLSIIPDYR TKVLAQFGER ASEVCLDDTV TTEKELDTVE VLKAIQKAKE VKSKLSNPEK KGGEDSRLSA APCIRPSSSP PTVAPASASL PQPILSNQGI MFVQEEALAS SLSSTDSLTP EHQPIAQGCS DSLESIPAGQ AASDDLRDVP GAVGGASPEH AEPEVQVVPG SGQIIFLPFT CIGYTATNQD FIQRLSTLIR QAIERQLPAW IEAANQREEG QGEQGEEEDE EEEEEEDVAE NRYFEMGPPD VEEEEGGGQG EEEEEEEEDE EAEEERLALE WALGADEDFL LEHIRILKVL WCFLIHVQGS IRQFAACLVL TDFGIAVFEI PHQESRGSSQ HILSSLRFVF CFPHGDLTEF GFLMPELCLV LKVRHSENTL FIISDAANLH EFHADLRSCF APQHMAMLCS PILYGSHTSL QEFLRQLLTF YKVAGGCQER SQGCFPVYLV YSDKRMVQTA AGDYSGNIEW ASCTLCSAVR RSCCAPSEAV KSAAIPYWLL LTPQHLNVIK ADFNPMPNRG THNCRNRNSF KLSRVPLSTV LLDPTRSCTQ PRGAFADGHV LELLVGYRFV TAIFVLPHEK FHFLRVYNQL RASLQDLKTV VIAKTPGTGG SPQGSFADGQ PAERRASNDQ RPQEVPAEAL APAPAEVPAP APAAASASGP AKTPAPAEAS TSALVPEETP VEAPAPPPAE APAQYPSEHL IQATSEENQI PSHLPACPSL RHVASLRGSA IIELFHSSIA EVENEELRHL MWSSVVFYQT PGLEVTACVL LSTKAVYFVL HDGLRRYFSE PLQDFWHQKN TDYNNSPFHI SQCFVLKLSD LQSVNVGLFD QHFRLTGSTP MQVVTCLTRD SYLTHCFLQH LMVVLSSLER TPSPEPVDKD FYSEFGNKTT GKMENYELIH SSRVKFTYPS EEEIGDLTFT VAQKMAEPEK APALSILLYV QAFQVGMPPP GCCRGPLRPK TLLLTSSEIF LLDEDCVHYP LPEFAKEPPQ RDRYRLDDGR RVRDLDRVLM GYQTYPQALT LVFDDVQGHD LMGSVTLDHF GEVPGGPARA SQGREVQWQV FVPSAESREK LISLLARQWE ALCGRELPVE LTG
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human NISCH Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Nischarin (NISCH)

Alternative Name: NISCH (NISCH Products)

Synonyms: NISCH Protein, IRAS Protein, 1200007D05Rik Protein, 3202002H23Rik Protein, AW494485 Protein, I-1 Protein, IR1 Protein, hIRAS Protein, nischarin Protein, nischarin S homeolog Protein, NISCH Protein, Cyan7822_6841 Protein, nisch.S Protein, nisch Protein, Nisch Protein

Background: Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla (RVLM) neurons that exhibited rilmenidine-evoked hypotension (By similarity). Blocking its activation with efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons (By similarity). Acts as a modulator of Rac-regulated signal transduction pathways (By similarity). Suppresses Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity (By similarity). Also blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response element and cyclin D1 promoter activation (By similarity). Inhibits also LIMK1 kinase activity by reducing LIMK1 'Tyr-508' phosphorylation (By similarity). Inhibits Rac-induced cell migration and invasion in breast and colon epithelial cells (By similarity). Inhibits lamellipodia formation, when overexpressed (By similarity). Plays a role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular endosomal structures. {ECO:0000250, ECO:0000269|PubMed:10882231, ECO:0000269|PubMed:12868002, ECO:0000269|PubMed:15028619, ECO:0000269|PubMed:15028621, ECO:0000269|PubMed:15475348}.

Molecular Weight: 167.5 kDa Including tag.

UniProt: Q9Y2I1