NFRKB Protein (AA 1-1299) (Strep Tag)

Catalog No. ABIN3094143

Product Details

Target: NFRKB (Nuclear Factor Related To KappaB Binding Protein (NFRKB))

Protein Type: Recombinant

Protein Characteristics: AA 1-1299

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This NFRKB protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MDSLDHMLTD PLELGPCGDG HGTRIMEDCL LGGTRVSLPE DLLEDPEIFF DVVSLSTWQE VLSDSQREHL QQFLPQFPED SAEQQNELIL ALFSGENFRF GNPLHIAQKL FRDGHFNPEV VKYRQLCFKS QYKRYLNSQQ QYFHRLLKQI LASRSDLLEM ARRSGPALPF RQKRPSPSRT PEEREWRTQQ RYLKVLREVK EECGDTALSS DEEDLSSWLP SSPARSPSPA VPLRVVPTLS TTDMKTADKV ELGDSDLKIM LKKHHEKRKH QPDHPDLLTG DLTLNDIMTR VNAGRKGSLA ALYDLAVLKK KVKEKEEKKK KKIKTIKSEA EDLAEPLSST EGVAPLSQAP SPLAIPAIKE EPLEDLKPCL GINEISSSFF SLLLEILLLE SQASLPMLEE RVLDWQSSPA SSLNSWFSAA PNWAELVLPA LQYLAGESRA VPSSFSPFVE FKEKTQQWKL LGQSQDNEKE LAALFQLWLE TKDQAFCKQE NEDSSDATTP VPRVRTDYVV RPSTGEEKRV FQEQERYRYS QPHKAFTFRM HGFESVVGPV KGVFDKETSL NKAREHSLLR SDRPAYVTIL SLVRDAAARL PNGEGTRAEI CELLKDSQFL APDVTSTQVN TVVSGALDRL HYEKDPCVKY DIGRKLWIYL HRDRSEEEFE RIHQAQAAAA KARKALQQKP KPPSKVKSSS KESSIKVLSS GPSEQSQMSL SDSSMPPTPV TPVTPTTPAL PAIPISPPPV SAVNKSGPST VSEPAKSSSG VLLVSSPTMP HLGTMLSPAS SQTAPSSQAA ARVVSHSGSA GLSQVRVVAQ PSLPAVPQQS GGPAQTLPQM PAGPQIRVPA TATQTKVVPQ TVMATVPVKA QTTAATVQRP GPGQTGLTVT SLPATASPVS KPATSSPGTS APSASTAAVI QNVTGQNIIK QVAITGQLGV KPQTGNSIPL TATNFRIQGK DVLRLPPSSI TTDAKGQTVL RITPDMMATL AKSQVTTVKL TQDLFGTGGN TTGKGISATL HVTSNPVHAA DSPAKASSAS APSSTPTGTT VVKVTPDLKP TEASSSAFRL MPALGVSVAD QKGKSTVASS EAKPAATIRI VQGLGVMPPK AGQTITVATH AKQGASVASG SGTVHTSAVS LPSMNAAVSK TVAVASGAAS TPISISTGAP TVRQVPVSTT VVSTSQAGKL PTRITVPLSV ISQPMKGKSV VTAPIIKGNL GANLSGLGRN IILTTMPAGT KLIAGNKPVS FLTAQQLQQL QQQGQATQVR IQTVPASHLQ QGTASGSSKA VSTVVVTTAP SPKQAPEQQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: NFRKB (Nuclear Factor Related To KappaB Binding Protein (NFRKB))

Alternative Name: NFRKB (NFRKB Products)

Synonyms: nfrkb Protein, MGC75871 Protein, NFRKB Protein, A530090G11Rik Protein, INO80G Protein, nuclear factor related to kappaB binding protein Protein, amyloid beta precursor like protein 2 Protein, nuclear factor related to kappa B binding protein Protein, nfrkb Protein, NFRKB Protein, APLP2 Protein, Nfrkb Protein

Background: Nuclear factor related to kappa-B-binding protein (DNA-binding protein R kappa-B) (INO80 complex subunit G),FUNCTION: Binds to the DNA consensus sequence 5'-GGGGAATCTCC-3'. {ECO:0000269|PubMed:18922472}., FUNCTION: Putative regulatory component of the chromatin remodeling INO80 complex which is involved in transcriptional regulation, DNA replication and probably DNA repair. Modulates the deubiquitinase activity of UCHL5 in the INO80 complex. {ECO:0000269|PubMed:18922472}.

Molecular Weight: 139.0 kDa

UniProt: Q6P4R8