SMEK1 Protein (AA 1-833) (Strep Tag)

Catalog No. ABIN3094413

Product Details

Target: SMEK1 (Serine/threonine-Protein Phosphatase 4 Regulatory Subunit 3A (SMEK1))

Protein Type: Recombinant

Protein Characteristics: AA 1-833

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SMEK1 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MTDTRRRVKV YTLNEDRQWD DRGTGHVSSG YVERLKGMSL LVRAESDGSL LLESKINPNT AYQKQQDTLI VWSEAENYDL ALSFQEKAGC DEIWEKICQV QGKDPSVDIT QDLVDESEEE RFDDMSSPGL ELPSCELSRL EEIAELVASS LPSPLRREKL ALALENEGYI KKLLELFHVC EDLENIEGLH HLYEIIKGIF LLNRTALFEV MFSEECIMDV IGCLEYDPAL SQPRKHREFL TKTAKFKEVI PISDPELKQK IHQTYRVQYI QDMVLPTPSV FEENMLSTLH SFIFFNKVEI VGMLQEDEKF LTDLFAQLTD EATDEEKRQE LVNFLKEFCA FSQTLQPQNR DAFFKTLSNM GILPALEVIL GMDDTQVRSA ATDIFSYLVE YNPSMVREFV MQEAQQNDDV SKKLTEQKIT SKDILLINLI IEHMICDTDP ELGGAVQLMG LLRTLVDPEN MLATANKTEK TEFLGFFYKH CMHVLTAPLL ANTTEDKPSK DDFQTAQLLA LVLELLTFCV EHHTYHIKNY IINKDILRRV LVLMASKHAF LALCALRFKR KIIGLKDEFY NRYIMKSFLF EPVVKAFLNN GSRYNLMNSA IIEMFEFIRV EDIKSLTAHV IENYWKALED VDYVQTFKGL KLRFEQQRER QDNPKLDSMR SILRNHRYRR DARTLEDEEE MWFNTDEDDM EDGEAVVSPS DKTKNDDDIM DPISKFMERK KLKESEEKEV LLKTNLSGRQ SPSFKLSLSS GTKTNLTSQS STTNLPGSPG SPGSPGSPGS PGSVPKNTSQ TAAITTKGGL VGLVDYPDDD EDDDEDEDKE DTLPLSKKAK FDS
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SMEK1 (Serine/threonine-Protein Phosphatase 4 Regulatory Subunit 3A (SMEK1))

Alternative Name: PPP4R3A (SMEK1 Products)

Synonyms: FLFL1 Protein, KIAA2010 Protein, PP4R3A Protein, smk-1 Protein, smk1 Protein, 1110034C04Rik Protein, BC064465 Protein, mKIAA2010 Protein, RGD1309059 Protein, protein phosphatase 4 regulatory subunit 3A Protein, protein phosphatase 4, regulatory subunit 3A Protein, PPP4R3A Protein, Ppp4r3a Protein

Background: Serine/threonine-protein phosphatase 4 regulatory subunit 3A (SMEK homolog 1),FUNCTION: Regulatory subunit of serine/threonine-protein phosphatase 4. May regulate the activity of PPP4C at centrosomal microtubule organizing centers. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AX phosphorylated on 'Ser-140' (gamma-H2AX) generated during DNA replication and required for DNA DSB repair. {ECO:0000269|PubMed:18614045}.

Molecular Weight: 95.4 kDa

UniProt: Q6IN85

Pathways: Regulation of Carbohydrate Metabolic Process