PHAX Protein (AA 2-394) (His tag)

Catalog No. ABIN3094543

Product Details

Target: PHAX (phosphorylated Adaptor For RNA Export (PHAX))

Protein Type: Recombinant

Protein Characteristics: AA 2-394

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This PHAX protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: ALEVGDMEDG QLSDSDSDMT VAPSDRPLQL PKVLGGDSAM RAFQNTATAC APVSHYRAVE SVDSSEESFS DSDDDSCLWK RKRQKCFNPP PKPEPFQFGQ SSQKPPVAGG KKINNIWGAV LQEQNQDAVA TELGILGMEG TIDRSRQSET YNYLLAKKLR KESQEHTKDL DKELDEYMHG GKKMGSKEEE NGQGHLKRKR PVKDRLGNRP EMNYKGRYEI TAEDSQEKVA DEISFRLQEP KKDLIARVVR IIGNKKAIEL LMETAEVEQN GGLFIMNGSR RRTPGGVFLN LLKNTPSISE EQIKDIFYIE NQKEYENKKA ARKRRTQVLG KKMKQAIKSL NFQEDDDTSR ETFASDTNEA LASLDESQEG HAEAKLEAEE AIEVDHSHDL DIF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human PHAX Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PHAX (phosphorylated Adaptor For RNA Export (PHAX))

Alternative Name: PHAX (PHAX Products)

Synonyms: RNUXA Protein, 2810055C14Rik Protein, 4933427L19Rik Protein, AU018701 Protein, AU018854 Protein, D18Ertd65e Protein, Rnuxa Protein, p55 Protein, phosphorylated adaptor for RNA export Protein, PHAX Protein, Phax Protein

Background: A phosphoprotein adapter involved in the XPO1-mediated U snRNA export from the nucleus. Bridge components required for U snRNA export, the cap binding complex (CBC)-bound snRNA on the one hand and the GTPase Ran in its active GTP-bound form together with the export receptor XPO1 on the other. Its phosphorylation in the nucleus is required for U snRNA export complex assembly and export, while its dephosphorylation in the cytoplasm causes export complex disassembly. It is recycled back to the nucleus via the importin alpha/beta heterodimeric import receptor. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Its compartmentalized phosphorylation cycle may also contribute to the directionality of export. Binds strongly to m7G-capped U1 and U5 small nuclear RNAs (snRNAs) in a sequence-unspecific manner and phosphorylation-independent manner (By similarity). Plays also a role in the biogenesis of U3 small nucleolar RNA (snoRNA). Involved in the U3 snoRNA transport from nucleoplasm to Cajal bodies. Binds strongly to m7G-capped U3, U8 and U13 precursor snoRNAs and weakly to trimethylated (TMG)-capped U3, U8 and U13 snoRNAs. Binds also to telomerase RNA. {ECO:0000250, ECO:0000269|PubMed:15574332, ECO:0000269|PubMed:15574333}.

Molecular Weight: 45.2 kDa Including tag.

UniProt: Q9H814

Pathways: Ribonucleoprotein Complex Subunit Organization