Pinin, Desmosome Associated Protein (PNN) (AA 2-717) protein (His tag)

Details for Product No. ABIN3094578
Protein Name
Protein Characteristics
AA 2-717
1
1
Origin
Human
1
1
Source
Insect Cells
1
1
Protein Type
Recombinant
Purification tag / Conjugate
This PNN protein is labelled with His tag.
Application
Crystallization (Crys), ELISA, SDS-PAGE (SDS), Western Blotting (WB)
Options
Sequence AVAVRTLQEQ LEKAKESLKN VDENIRKLTG RDPNDVRPIQ ARLLALSGPG GGRGRGSLLL RRGFSDSGGG PPAKQRDLEG AVSRLGGERR TRRESRQESD PEDDDVKKPA LQSSVVATSK ERTRRDLIQD QNMDEKGKQR NRRIFGLLMG TLQKFKQEST VATERQKRRQ EIEQKLEVQA EEERKQVENE RRELFEERRA KQTELRLLEQ KVELAQLQEE WNEHNAKIIK YIRTKTKPHL FYIPGRMCPA TQKLIEESQR KMNALFEGRR IEFAEQINKM EARPRRQSMK EKEHQVVRNE EQKAEQEEGK VAQREEELEE TGNQHNDVEI EEAGEEEEKE IAIVHSDAEK EQEEEEQKQE MEVKMEEETE VRESEKQQDS QPEEVMDVLE MVENVKHVIA DQEVMETNRV ESVEPSENEA SKELEPEMEF EIEPDKECKS LSPGKENVSA LDMEKESEEK EEKESEPQPE PVAQPQPQSQ PQLQLQSQSQ PVLQSQPPSQ PEDLSLAVLQ PTPQVTQEQG HLLPERKDFP VESVKLTEVP VEPVLTVHPE SKSKTKTRSR SRGRARNKTS KSRSRSSSSS SSSSSSTSSS SGSSSSSGSS SSRSSSSSSS STSGSSSRDS SSSTSSSSES RSRSRGRGHN RDRKHRRSVD RKRRDTSGLE RSHKSSKGGS SRDTKGSKDK NSRSDRKRSI SESSRSGKRS SRSERDRKSD RKDKRR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
Characteristics
  • Made in Germany - from design to production - by highly experienced protein experts.
  • Human PNN Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
  • State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification Two step purification of proteins expressed in bacterial culture:
  1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
  2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
Purity >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
Sterility 0.22 μm filtered
Endotoxin Level Protein is endotoxin free.
Grade Crystallography grade
Protein Name
Background Transcriptional activator binding to the E-box 1 core sequence of the E-cadherin promoter gene, the core-binding sequence is 5'CAGGTG-3'. Capable of reversing CTBP1-mediated transcription repression. Auxiliary component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Participates in the regulation of alternative pre-mRNA splicing. Associates to spliced mRNA within 60 nt upstream of the 5'-splice sites. Component of the PSAP complex which binds RNA in a sequence-independent manner and is proposed to be recruited to the EJC prior to or during the splicing process and to regulate specific excision of introns in specific transcription subsets. Involved in the establishment and maintenance of epithelia cell-cell adhesion. Potential tumor suppressor for renal cell carcinoma. {ECO:0000269|PubMed:12051732, ECO:0000269|PubMed:14517304, ECO:0000269|PubMed:15542832, ECO:0000269|PubMed:15735603, ECO:0000269|PubMed:22388736}.
Molecular Weight 82.4 kDa Including tag.
UniProt Q9H307
Application Notes In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
Comment

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions For Research Use only
Format Liquid
Buffer 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -80 °C
Storage Comment Store at -80°C.
Expiry Date Unlimited (if stored properly)
Supplier Images
Image no. 1 for Pinin, Desmosome Associated Protein (PNN) (AA 2-717) protein (His tag) (ABIN3094578) „Crystallography Grade“ protein due to multi-step, protein-specific purification process