PRDM14 Protein (AA 1-571) (Strep Tag)

Catalog No. ABIN3094769

Product Details

Target: PRDM14 (PR Domain Containing 14 (PRDM14))

Protein Type: Recombinant

Protein Characteristics: AA 1-571

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This PRDM14 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MALPRPSEAV PQDKVCYPPE SSPQNLAAYY TPFPSYGHYR NSLATVEEDF QPFRQLEAAA SAAPAMPPFP FRMAPPLLSP GLGLQREPLY DLPWYSKLPP WYPIPHVPRE VPPFLSSSHE YAGASSEDLG HQIIGGDNES GPCCGPDTLI PPPPADASLL PEGLRTSQLL PCSPSKQSED GPKPSNQEGK SPARFQFTEE DLHFVLYGVT PSLEHPASLH HAISGLLVPP DSSGSDSLPQ TLDKDSLQLP EGLCLMQTVF GEVPHFGVFC SSFIAKGVRF GPFQGKVVNA SEVKTYGDNS VMWEIFEDGH LSHFIDGKGG TGNWMSYVNC ARFPKEQNLV AVQCQGHIFY ESCKEIHQNQ ELLVWYGDCY EKFLDIPVSL QVTEPGKQPS GPSEESAEGY RCERCGKVFT YKYYRDKHLK YTPCVDKGDR KFPCSLCKRS FEKRDRLRIH ILHVHEKHRP HKCSTCGKCF SQSSSLNKHM RVHSGDRPYQ CVYCTKRFTA SSILRTHIRQ HSGEKPFKCK YCGKSFASHA AHDSHVRRSH KEDDGCSCSI CGKIFSDQET FYSHMKFHED Y
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: PRDM14 (PR Domain Containing 14 (PRDM14))

Alternative Name: PRDM14 (PRDM14 Products)

Synonyms: PFM11 Protein, PRDM14 Protein, prmd14 Protein, fc16b12 Protein, wu:fc16b12 Protein, PR/SET domain 14 Protein, PR domain containing 14 Protein, PRDM14 Protein, Prdm14 Protein, prdm14 Protein

Background: PR domain zinc finger protein 14 (EC 2.1.1.-) (PR domain-containing protein 14),FUNCTION: Transcription factor that has both positive and negative roles on transcription. Required for the maintenance of embryonic stem cell identity and the reacquisition of pluripotency in somatic cells. May play an essential role in germ cell development at 2 levels: the reacquisition of potential pluripotency, including SOX2 up-regulation, and successful epigenetic reprogramming, characterized by EHMT1 repression. Its association with CBFA2T2 is required for the functions in pluripotency and germ cell formation (By similarity). Directly up-regulates the expression of pluripotency gene POU5F1 through its proximal enhancer. Binds to the DNA consensus sequence 5'-GGTC[TC]CTAA-3'. {ECO:0000250|UniProtKB:E9Q3T6, ECO:0000269|PubMed:17942894, ECO:0000269|PubMed:20953172}.

Molecular Weight: 64.1 kDa

UniProt: Q9GZV8