RBM8A Protein (AA 2-174) (His tag)

Catalog No. ABIN3094969

Product Details

Target: RBM8A (RNA Binding Motif Protein 8A (RBM8A))

Protein Type: Recombinant

Protein Characteristics: AA 2-174

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This RBM8A protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: ADVLDLHEAG GEDFAMDEDG DESIHKLKEK AKKRKGRGFG SEEGSRARMR EDYDSVEQDG DEPGPQRSVE GWILFVTGVH EEATEEDIHD KFAEYGEIKN IHLNLDRRTG YLKGYTLVEY ETYKEAQAAM EGLNGQDLMG QPISVDWCFV RGPPKGKRRG GRRRSRSPDR RRR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human RBM8A Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: RBM8A (RNA Binding Motif Protein 8A (RBM8A))

Alternative Name: RBM8A (RBM8A Products)

Synonyms: BOV-1A Protein, BOV-1B Protein, BOV-1C Protein, C1DELq21.1 Protein, DEL1q21.1 Protein, MDS014 Protein, RBM8 Protein, RBM8B Protein, TAR Protein, Y14 Protein, ZNRP Protein, ZRNP1 Protein, 2310057C03Rik Protein, AA673428 Protein, Rbm8 Protein, RBM8A Protein, rbm8 Protein, rbm8a Protein, rnp-5 Protein, fb34f03 Protein, wu:fb34f03 Protein, zgc:110525 Protein, rbm8-A Protein, rbm8a-A Protein, rbm8-B Protein, RNA binding motif protein 8A Protein, RNA binding motif protein 8a Protein, RNA binding motif protein 8A S homeolog Protein, RNA binding motif protein 8A L homeolog Protein, RBM8A Protein, Rbm8a Protein, rbm8a Protein, rbm8a.S Protein, rbm8a.L Protein

Background: Core component of the splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junctions on mRNAs. The EJC is a dynamic structure consisting of core proteins and several peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. The EJC marks the position of the exon-exon junction in the mature mRNA for the gene expression machinery and the core components remain bound to spliced mRNAs throughout all stages of mRNA metabolism thereby influencing downstream processes including nuclear mRNA export, subcellular mRNA localization, translation efficiency and nonsense-mediated mRNA decay (NMD). The MAGOH-RBM8A heterodimer inhibits the ATPase activity of EIF4A3, thereby trapping the ATP-bound EJC core onto spliced mRNA in a stable conformation. The MAGOH-RBM8A heterodimer interacts with the EJC key regulator PYM1 leading to EJC disassembly in the cytoplasm and translation enhancement of EJC-bearing spliced mRNAs by recruiting them to the ribosomal 48S preinitiation complex. Its removal from cytoplasmic mRNAs requires translation initiation from EJC-bearing spliced mRNAs. Associates preferentially with mRNAs produced by splicing. Does not interact with pre-mRNAs, introns, or mRNAs produced from intronless cDNAs. Associates with both nuclear mRNAs and newly exported cytoplasmic mRNAs. The MAGOH-RBM8A heterodimer is a component of the nonsense mediated decay (NMD) pathway. Involved in the splicing modulation of BCL2L1/Bcl-X (and probably other apoptotic genes), specifically inhibits formation of proapoptotic isoforms such as Bcl-X(S), the function is different from the established EJC assembly. {ECO:0000269|PubMed:12121612, ECO:0000269|PubMed:12718880, ECO:0000269|PubMed:12730685, ECO:0000269|PubMed:16209946, ECO:0000269|PubMed:19409878, ECO:0000269|PubMed:22203037}.

Molecular Weight: 20.7 kDa Including tag.

UniProt: Q9Y5S9