RPRD1B Protein (AA 2-326) (His tag)

Catalog No. ABIN3095124

Product Details

Target: RPRD1B (Regulation of Nuclear Pre-mRNA Domain Containing 1B (RPRD1B))

Protein Type: Recombinant

Protein Characteristics: AA 2-326

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This RPRD1B protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Crystallization (Crys)

Sequence: SSFSESALEK KLSELSNSQQ SVQTLSLWLI HHRKHAGPIV SVWHRELRKA KSNRKLTFLY LANDVIQNSK RKGPEFTREF ESVLVDAFSH VAREADEGCK KPLERLLNIW QERSVYGGEF IQQLKLSMED SKSPPPKATE EKKSLKRTFQ QIQEEEDDDY PGSYSPQDPS AGPLLTEELI KALQDLENAA SGDATVRQKI ASLPQEVQDV SLLEKITDKE AAERLSKTVD EACLLLAEYN GRLAAELEDR RQLARMLVEY TQNQKDVLSE KEKKLEEYKQ KLARVTQVRK ELKSHIQSLP DLSLLPNVTG GLAPLPSAGD LFSTD
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human RPRD1B Protein (raised in E. Coli) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in bacterial culture:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Endotoxin has not been removed. Please contact us if you require endotoxin removal.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: RPRD1B (Regulation of Nuclear Pre-mRNA Domain Containing 1B (RPRD1B))

Alternative Name: RPRD1B (RPRD1B Products)

Synonyms: fb23h08 Protein, zgc:55687 Protein, wu:fb23h08 Protein, rprd1ba Protein, MGC130705 Protein, C13H20ORF77 Protein, C20orf77 Protein, CREPT Protein, NET60 Protein, dJ1057B20.2 Protein, 2610304G08Rik Protein, 2810446G03Rik Protein, Crept Protein, RGD1304782 Protein, regulation of nuclear pre-mRNA domain containing 1B Protein, regulation of nuclear pre-mRNA domain containing 1B L homeolog Protein, rprd1b Protein, rprd1b.L Protein, RPRD1B Protein, Rprd1b Protein

Background: Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation. {ECO:0000269|PubMed:22231121, ECO:0000269|PubMed:22264791}.

Molecular Weight: 37.7 kDa Including tag.

UniProt: Q9NQG5