RPS27A Protein (AA 77-156) (His tag)

Catalog No. ABIN3095129

Product Details

Target: RPS27A (Ribosomal Protein S27a (RPS27A))

Protein Type: Recombinant

Protein Characteristics: AA 77-156

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This RPS27A protein is labelled with His tag.

Application: ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)

Sequence: AKKRKKKSYT TPKKNKHKRK KVKLAVLKYY KVDENGKISR LRRECPSDEC GAGVFMASHF DRHYCGKCCL TYCFNKPEDK
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human RPS27A Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: RPS27A (Ribosomal Protein S27a (RPS27A))

Alternative Name: RPS27A (RPS27A Products)

Synonyms: CEP80 Protein, S27A Protein, UBA80 Protein, UBC Protein, UBCEP1 Protein, UBCEP80 Protein, 0610006J14Rik Protein, Uba52 Protein, Ubb Protein, Ubc Protein, CG5271 Protein, DUb80 Protein, Dmel\\CG5271 Protein, Dub80 Protein, UB3-D Protein, Ubi-f80 Protein, Ubi-m Protein, Ubiq Protein, l(2)04820 Protein, l(2)31Ei Protein, mfs(2)31 Protein, mfs(2)48 Protein, mfs48 Protein, Ubiz Protein, hm:zeh0386 Protein, ik:tdsubc_1f2 Protein, tdsubc_1f2 Protein, xx:tdsubc_1f2 Protein, zgc:66168 Protein, rps27a Protein, rps27a.L Protein, ribosomal protein S27a Protein, ribosomal protein S27A Protein, Ribosomal protein S27A Protein, ubiquitin-40S ribosomal protein S27a Protein, ribosomal protein S27a S homeolog Protein, RPS27A Protein, Rps27a Protein, RpS27A Protein, rps27a Protein, LOC105381543 Protein, rps27a.S Protein

Background: Ubiquitin: Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair, Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation, Lys-29-linked is involved in lysosomal degradation, Lys-33-linked is involved in kinase modification, Lys-48-linked is involved in protein degradation via the proteasome, Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling., 40S Ribosomal protein S27a: Component of the 40S subunit of the ribosome.

Molecular Weight: 10.4 kDa Including tag.

UniProt: P62979

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, Toll-Like Receptors Cascades, Synthesis of DNA, EGFR Downregulation