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Reticulon 3 Protein (RTN3) (AA 2-1032) (His tag)

Crystallography grade RTN3 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3095135
  • Target See all Reticulon 3 (RTN3) Proteins
    Reticulon 3 (RTN3)
    Protein Type
    Recombinant
    Protein Characteristics
    AA 2-1032
    Origin
    • 4
    • 2
    • 1
    • 1
    Human
    Source
    • 3
    • 2
    • 2
    • 1
    Insect Cells
    Purification tag / Conjugate
    This Reticulon 3 protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    AEPSAATQSH SISSSSFGAE PSAPGGGGSP GACPALGTKS CSSSCADSFV SSSSSQPVSL FSTSQEGLSS LCSDEPSSEI MTSSFLSSSE IHNTGLTILH GEKSHVLGSQ PILAKEGKDH LDLLDMKKME KPQGTSNNVS DSSVSLAAGV HCDRPSIPAS FPEHPAFLSK KIGQVEEQID KETKNPNGVS SREAKTALDA DDRFTLLTAQ KPPTEYSKVE GIYTYSLSPS KVSGDDVIEK DSPESPFEVI IDKAAFDKEF KDSYKESTDD FGSWSVHTDK ESSEDISETN DKLFPLRNKE AGRYPMSALL SRQFSHTNAA LEEVSRCVND MHNFTNEILT WDLVPQVKQQ TDKSSDCITK TTGLDMSEYN SEIPVVNLKT STHQKTPVCS IDGSTPITKS TGDWAEASLQ QENAITGKPV PDSLNSTKEF SIKGVQGNMQ KQDDTLAELP GSPPEKCDSL GSGVATVKVV LPDDHLKDEM DWQSSALGEI TEADSSGESD DTVIEDITAD TSFENNKIQA EKPVSIPSAV VKTGEREIKE IPSCEREEKT SKNFEELVSD SELHQDQPDI LGRSPASEAA CSKVPDTNVS LEDVSEVAPE KPITTENPKL PSTVSPNVFN ETEFSLNVTT SAYLESLHGK NVKHIDDSSP EDLIAAFTET RDKGIVDSER NAFKAISEKM TDFKTTPPVE VLHENESGGS EIKDIGSKYS EQSKETNGSE PLGVFPTQGT PVASLDLEQE QLTIKALKEL GERQVEKSTS AQRDAELPSE EVLKQTFTFA PESWPQRSYD ILERNVKNGS DLGISQKPIT IRETTRVDAV SSLSKTELVK KHVLARLLTD FSVHDLIFWR DVKKTGFVFG TTLIMLLSLA AFSVISVVSY LILALLSVTI SFRIYKSVIQ AVQKSEEGHP FKAYLDVDIT LSSEAFHNYM NAAMVHINRA LKLIIRLFLV EDLVDSLKLA VFMWLMTYVG AVFNGITLLI LAELLIFSVP IVYEKYKTQI DHYVGIARDQ TKSIVEKIQA KLPGIAKKKA E
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human RTN3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product RTN3 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    Reticulon 3 (RTN3)
    Alternative Name
    RTN3 (RTN3 Products)
    Synonyms
    ASYIP Protein, HAP Protein, NSPL2 Protein, NSPLII Protein, RTN3-A1 Protein, MGC56439 Protein, zgc:56439 Protein, zgc:77630 Protein, RTN3 Protein, hap Protein, asyip Protein, nspl2 Protein, xrtn3 Protein, nsplii Protein, rtn3-a1 Protein, RTN3w Protein, rtn3 Protein, XRTN3 Protein, rtn3-a Protein, rtn3-b Protein, reticulon 3 Protein, reticulon 3 L homeolog Protein, reticulon-3 Protein, reticulon 3 S homeolog Protein, RTN3 Protein, Rtn3 Protein, rtn3 Protein, rnt3 Protein, rtn3.L Protein, LOC107131155 Protein, rtn3.S Protein
    Background
    May be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress. In case of enteroviruses infection, RTN3 may be involved in the viral replication or pathogenesis. Induces the formation of endoplasmic reticulum tubules (PubMed:25612671). {ECO:0000269|PubMed:15286784, ECO:0000269|PubMed:16054885, ECO:0000269|PubMed:17031492, ECO:0000269|PubMed:17191123, ECO:0000269|PubMed:25612671}.
    Molecular Weight
    113.4 kDa Including tag.
    UniProt
    O95197
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