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SCARA5 Protein (AA 82-495) (His tag)

Crystallography grade SCARA5 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Catalog No. ABIN3095200
  • Target See all SCARA5 Proteins
    SCARA5 (Scavenger Receptor Class A, Member 5 (SCARA5))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 82-495
    Origin
    • 3
    • 2
    • 1
    • 1
    Human
    Source
    • 3
    • 2
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This SCARA5 protein is labelled with His tag.
    Application
    SDS-PAGE (SDS), Western Blotting (WB), ELISA, Crystallization (Crys)
    Sequence
    SRPRSSPDDL KALTRNVNRL NESFRDLQLR LLQAPLQADL TEQVWKVQDA LQNQSDSLLA LAGAVQRLEG ALWGLQAQAV QTEQAVALLR DRTGQQSDTA QLELYQLQVE SNSSQLLLRR HAGLLDGLAR RVGILGEELA DVGGVLRGLN HSLSYDVALH RTRLQDLRVL VSNASEDTRR LRLAHVGMEL QLKQELAMLN AVTEDLRLKD WEHSIALRNI SLAKGPPGPK GDQGDEGKEG RPGIPGLPGL RGLPGERGTP GLPGPKGDDG KLGATGPMGM RGFKGDRGPK GEKGEKGDRA GDASGVEAPM MIRLVNGSGP HEGRVEVYHD RRWGTVCDDG WDKKDGDVVC RMLGFRGVEE VYRTARFGQG TGRIWMDDVA CKGTEETIFR CSFSKWGVTN CGHAEDASVT CNRH
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human SCARA5 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    SCARA5 (Scavenger Receptor Class A, Member 5 (SCARA5))
    Alternative Name
    SCARA5 (SCARA5 Products)
    Synonyms
    4932433F15Rik Protein, 4933425F03Rik Protein, AV278087 Protein, Tesr Protein, RGD1306539 Protein, NET33 Protein, scara5 Protein, scavenger receptor class A, member 5 Protein, scavenger receptor class A member 5 Protein, scavenger receptor class A, member 5 (putative) Protein, Scara5 Protein, SCARA5 Protein, scara5 Protein
    Background
    Ferritin receptor that mediates non-transferrin-dependent delivery of iron. Mediates cellular uptake of ferritin-bound iron by stimulating ferritin endocytosis from the cell surface with consequent iron delivery within the cell. Delivery of iron to cells by ferritin is required for the development of specific cell types, suggesting the existence of cell type-specific mechanisms of iron traffic in organogenesis, which alternatively utilize transferrin or non-transferrin iron delivery pathways. Ferritin mediates iron uptake in capsule cells of the developing kidney. Binds preferrentially ferritin light chain (FTL) compared to heavy chain (FTH1) (By similarity). {ECO:0000250}.
    Molecular Weight
    46.1 kDa Including tag.
    UniProt
    Q6ZMJ2
    Pathways
    Transition Metal Ion Homeostasis
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