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AHCYL1 Protein (AA 2-530) (His tag)

Crystallography grade AHCYL1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Catalog No. ABIN3095272
  • Target See all AHCYL1 Proteins
    AHCYL1 (Adenosylhomocysteinase-Like 1 (AHCYL1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 2-530
    Origin
    • 4
    • 1
    Human
    Source
    • 2
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This AHCYL1 protein is labelled with His tag.
    Application
    Western Blotting (WB), SDS-PAGE (SDS), ELISA, Crystallization (Crys)
    Sequence
    SMPDAMPLPG VGEELKQAKE IEDAEKYSFM ATVTKAPKKQ IQFADDMQEF TKFPTKTGRR SLSRSISQSS TDSYSSAASY TDSSDDEVSP REKQQTNSKG SSNFCVKNIK QAEFGRREIE IAEQDMSALI SLRKRAQGEK PLAGAKIVGC THITAQTAVL IETLCALGAQ CRWSACNIYS TQNEVAAALA EAGVAVFAWK GESEDDFWWC IDRCVNMDGW QANMILDDGG DLTHWVYKKY PNVFKKIRGI VEESVTGVHR LYQLSKAGKL CVPAMNVNDS VTKQKFDNLY CCRESILDGL KRTTDVMFGG KQVVVCGYGE VGKGCCAALK ALGAIVYITE IDPICALQAC MDGFRVVKLN EVIRQVDVVI TCTGNKNVVT REHLDRMKNS CIVCNMGHSN TEIDVTSLRT PELTWERVRS QVDHVIWPDG KRVVLLAEGR LLNLSCSTVP TFVLSITATT QALALIELYN APEGRYKQDV YLLPKKMDEY VASLHLPSFD AHLTELTDDQ AKYLGLNKNG PFKPNYYRY
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human AHCYL1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product AHCYL1 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    AHCYL1 (Adenosylhomocysteinase-Like 1 (AHCYL1))
    Alternative Name
    AHCYL1 (AHCYL1 Products)
    Synonyms
    irbit Protein, DCAL Protein, IRBIT Protein, PRO0233 Protein, XPVKONA Protein, cb586 Protein, wu:fj66f02 Protein, 1110034F20Rik Protein, AA409031 Protein, AA414901 Protein, Ahcy-rs3 Protein, Irbit Protein, adenosylhomocysteinase like 1 Protein, adenosylhomocysteinase like 1 S homeolog Protein, adenosylhomocysteinase-like 1 Protein, S-adenosylhomocysteine hydrolase-like 1 Protein, AHCYL1 Protein, ahcyl1 Protein, ahcyl1.S Protein, Ahcyl1 Protein
    Background
    Multifaceted cellular regulator which coordinates several essential cellular functions including regulation of epithelial HCO3(-) and fluid secretion, mRNA processing and DNA replication. Regulates ITPR1 sensitivity to inositol 1,4,5-trisphosphate competing for the common binding site and acting as endogenous 'pseudoligand' whose inhibitory activity can be modulated by its phosphorylation status. In the pancreatic and salivary ducts, at resting state, attenuates inositol 1,4,5-trisphosphate-induced calcium release by interacting with ITPR1 (PubMed:16793548). When extracellular stimuli induce ITPR1 phosphorylation or inositol 1,4,5-trisphosphate production, dissociates of ITPR1 to interact with CFTR and SLC26A6 mediating their synergistic activation by calcium and cAMP that stimulates the epithelial secretion of electrolytes and fluid (By similarity). Also activates basolateral SLC4A4 isoform 1 to coordinate fluid and HCO3(-) secretion (PubMed:16769890). Inhibits the effect of STK39 on SLC4A4 and CFTR by recruiting PP1 phosphatase which activates SLC4A4, SLC26A6 and CFTR through dephosphorylation (By similarity). Mediates the induction of SLC9A3 surface expression produced by Angiotensin-2 (PubMed:20584908). Depending on the cell type, activates SLC9A3 in response to calcium or reverses SLC9A3R2-dependent calcium inhibition (PubMed:18829453). May modulate the polyadenylation state of specific mRNAs, both by controlling the subcellular location of FIP1L1 and by inhibiting PAPOLA activity, in response to a stimulus that alters its phosphorylation state (PubMed:19224921). Acts as a (dATP)-dependent inhibitor of ribonucleotide reductase large subunit RRM1, controlling the endogenous dNTP pool and ensuring normal cell cycle progression (PubMed:25237103). {ECO:0000250|UniProtKB:B5DFN2, ECO:0000250|UniProtKB:Q80SW1, ECO:0000269|PubMed:16769890, ECO:0000269|PubMed:16793548, ECO:0000269|PubMed:18829453, ECO:0000269|PubMed:19224921, ECO:0000269|PubMed:20584908, ECO:0000269|PubMed:25237103}.
    Molecular Weight
    59.8 kDa Including tag.
    UniProt
    O43865
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