SLC4A8 Protein (AA 1-1093) (Strep Tag)

Catalog No. ABIN3095331

Product Details

Target: SLC4A8 (Solute Carrier Family 4, Sodium Bicarbonate Cotransporter, Member 8 (SLC4A8))

Protein Type: Recombinant

Protein Characteristics: AA 1-1093

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SLC4A8 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MPAAGSNEPD GVLSYQRPDE EAVVDQGGTS TILNIHYEKE ELEGHRTLYV GVRMPLGRQS HRHHRTHGQK HRRRGRGKGA SQGEEGLEAL AHDTPSQRVQ FILGTEEDEE HVPHELFTEL DEICMKEGED AEWKETARWL KFEEDVEDGG ERWSKPYVAT LSLHSLFELR SCLINGTVLL DMHANSIEEI SDLILDQQEL SSDLNDSMRV KVREALLKKH HHQNEKKRNN LIPIVRSFAE VGKKQSDPHL MDKHGQTVSP QSVPTTNLEV KNGVNCEHSP VDLSKVDLHF MKKIPTGAEA SNVLVGEVDI LDRPIVAFVR LSPAVLLSGL TEVPIPTRFL FILLGPVGKG QQYHEIGRSM ATIMTDEIFH DVAYKAKERD DLLAGIDEFL DQVTVLPPGE WDPSIRIEPP KNVPSQEKRK MPGVPNGNVC HIEQEPHGGH SGPELQRTGR LFGGLVLDIK RKAPWYWSDY RDALSLQCLA SFLFLYCACM SPVITFGGLL GEATEGRISA IESLFGASMT GIAYSLFAGQ ALTILGSTGP VLVFEKILFK FCKDYALSYL SLRACIGLWT AFLCIVLVAT DASSLVCYIT RFTEEAFASL ICIIFIYEAI EKLIHLAETY PIHMHSQLDH LSLYYCRCTL PENPNNHTLQ YWKDHNIVTA EVHWANLTVS ECQEMHGEFM GSACGHHGPY TPDVLFWSCI LFFTTFILSS TLKTFKTSRY FPTRVRSMVS DFAVFLTIFT MVIIDFLIGV PSPKLQVPSV FKPTRDDRGW IINPIGPNPW WTVIAAIIPA LLCTILIFMD QQITAVIINR KEHKLKKGCG YHLDLLMVAI MLGVCSIMGL PWFVAATVLS ITHVNSLKLE SECSAPGEQP KFLGIREQRV TGLMIFVLMG CSVFMTAILK FIPMPVLYGV FLYMGVSSLQ GIQFFDRLKL FGMPAKHQPD FIYLRHVPLR KVHLFTLIQL TCLVLLWVIK ASPAAIVFPM MVLALVFVRK VMDLCFSKRE LSWLDDLMPE SKKKKLDDAK KKAKEEEEAE KMLEIGGDKF PLESRKLLSS PGKNISCRCD PSEINISDEM PKTTVWKALS MNSGNAKEKS LFN
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SLC4A8 (Solute Carrier Family 4, Sodium Bicarbonate Cotransporter, Member 8 (SLC4A8))

Alternative Name: SLC4A8 (SLC4A8 Products)

Synonyms: SLC4A8 Protein, AW493845 Protein, C230026C11 Protein, NDCBE Protein, kNBC-3 Protein, NBC3 Protein, KNBC3 Protein, solute carrier family 4, sodium bicarbonate cotransporter, member 8 S homeolog Protein, solute carrier family 4 member 8 Protein, solute carrier family 4, sodium bicarbonate cotransporter, member 8 Protein, solute carrier family 4 (anion exchanger), member 8 Protein, slc4a8.S Protein, SLC4A8 Protein, slc4a8 Protein, Slc4a8 Protein

Background: Electroneutral sodium bicarbonate exchanger 1 (Electroneutral Na(+)-driven Cl-HCO3 exchanger) (Solute carrier family 4 member 8) (k-NBC3),FUNCTION: Mediates electroneutral sodium- and carbonate-dependent chloride-HCO3(-) exchange with a Na(+):HCO3(-) stoichiometry of 2:1 (PubMed:18577713). Plays a major role in pH regulation in neurons (By similarity). Mediates sodium reabsorption in the renal cortical collecting ducts (By similarity). {ECO:0000250|UniProtKB:Q8JZR6, ECO:0000269|PubMed:18577713}.

Molecular Weight: 122.9 kDa

UniProt: Q2Y0W8