SSH2 Protein (AA 1-1423) (Strep Tag)

Catalog No. ABIN3095647

Product Details

Target: SSH2 (Slingshot Homolog 2 (SSH2))

Protein Type: Recombinant

Protein Characteristics: AA 1-1423

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This SSH2 protein is labelled with Strep Tag.

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA

Sequence: MALVTVQRSP TPSTTSSPCA SEADSGEEEC RSQPRSISES FLTVKGAALF LPRGNGSSTP RISHRRNKHA GDLQQHLQAM FILLRPEDNI RLAVRLESTY QNRTRYMVVV STNGRQDTEE SIVLGMDFSS NDSSTCTMGL VLPLWSDTLI HLDGDGGFSV STDNRVHIFK PVSVQAMWSA LQSLHKACEV ARAHNYYPGS LFLTWVSYYE SHINSDQSSV NEWNAMQDVQ SHRPDSPALF TDIPTERERT ERLIKTKLRE IMMQKDLENI TSKEIRTELE MQMVCNLREF KEFIDNEMIV ILGQMDSPTQ IFEHVFLGSE WNASNLEDLQ NRGVRYILNV TREIDNFFPG VFEYHNIRVY DEEATDLLAY WNDTYKFISK AKKHGSKCLV HCKMGVSRSA STVIAYAMKE YGWNLDRAYD YVKERRTVTK PNPSFMRQLE EYQGILLASK QRHNKLWRSH SDSDLSDHHE PICKPGLELN KKDITTSADQ IAEVKTMESH PPIPPVFVEH MVPQDANQKG LCTKERMICL EFTSREFHAG QIEDELNLND INGCSSGCCL NESKFPLDNC HASKALIQPG HVPEMANKFP DLTVEDLETD ALKADMNVHL LPMEELTSPL KDPPMSPDPE SPSPQPSCQT EISDFSTDRI DFFSALEKFV ELSQETRSRS FSHSRMEELG GGRNESCRLS VVEVAPSKVT ADDQRSSSLS NTPHASEESS MDEEQSKAIS ELVSPDIFMQ SHSENAISVK EIVTEIESIS QGVGQIQLKG DILPNPCHTP KKNSIHELLL ERAQTPENKP GHMEQDEDSC TAQPELAKDS GMCNPEGCLT THSSIADLEE GEPAEGEQEL QGSGMHPGAK WYPGSVRRAT LEFEERLRQE QEHHGAAPTC TSLSTRKNSK NDSSVADLAP KGKSDEAPPE HSFVLKEPEM SKGKGKYSGS EAGSLSHSEQ NATVPAPRVL EFDHLPDPQE GPGSDTGTQQ EGVLKDLRTV IPYQESETQA VPLPLPKRVE IIEYTHIVTS PNHTGPGSEI ATSEKSGEQG LRKVNMEKSV TVLCTLDENL NRTLDPNQVS LHPQVLPLPH SSSPEHNRPT DHPTSILSSP EDRGSSLSTA LETAAPFVSH TTHLLSASLD YLHPQTMVHL EGFTEQSSTT DEPSAEQVSW EESQESPLSS GSEVPYKDSQ LSSADLSLIS KLGDNTGELQ EKMDPLPVAC RLPHSSSSEN IKSLSHSPGV VKERAKEIES RVVFQAGLTK PSQMRRSASL AKLGYLDLCK DCLPEREPAS CESPHLKLLQ PFLRTDSGMH AMEDQESLEN PGAPHNPEPT KSFVEQLTTT ECIVQSKPVE RPLVQYAKEF GSSQQYLLPR AGLELTSSEG GLPVLQTQGL QCACPAPGLA VAPRQQHGRT HPLRRLKKAN DKKRTTNPFY NTM
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: SSH2 (Slingshot Homolog 2 (SSH2))

Alternative Name: SSH2 (SSH2 Products)

Synonyms: BB104748 Protein, BC030940 Protein, SSH-2 Protein, mSSH-2L Protein, SSH-2L Protein, ssh2 Protein, zgc:175142 Protein, slingshot homolog 2 (Drosophila) Protein, slingshot protein phosphatase 2 Protein, slingshot protein phosphatase 2 L homeolog Protein, slingshot protein phosphatase 2b Protein, Ssh2 Protein, SSH2 Protein, ssh2.L Protein, ssh2b Protein

Background: Protein phosphatase Slingshot homolog 2 (EC 3.1.3.16) (EC 3.1.3.48) (SSH-like protein 2) (SSH-2L) (hSSH-2L),FUNCTION: Protein phosphatase which regulates actin filament dynamics. Dephosphorylates and activates the actin binding/depolymerizing factor cofilin, which subsequently binds to actin filaments and stimulates their disassembly. Inhibitory phosphorylation of cofilin is mediated by LIMK1, which may also be dephosphorylated and inactivated by this protein (PubMed:11832213). Required for spermatogenesis (By similarity). Involved in acrosome biogenesis, probably by regulating cofilin-mediated actin cytoskeleton remodeling during proacrosomal vesicle fusion and/or Golgi to perinuclear vesicle trafficking (By similarity). {ECO:0000250|UniProtKB:Q5SW75, ECO:0000269|PubMed:11832213}.

Molecular Weight: 158.2 kDa

UniProt: Q76I76