TDRD3 Protein (AA 1-651) (His tag)
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- Target See all TDRD3 Proteins
- TDRD3 (Tudor Domain Containing 3 (TDRD3))
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-651
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Origin
- Human
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Source
- Insect Cells
- Purification tag / Conjugate
- This TDRD3 protein is labelled with His tag.
- Application
- SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA
- Sequence
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MLRLQMTDGH ISCTAVEFSY MSKISLNTPP GTKVKLSGIV DIKNGFLLLN DSNTTVLGGE VEHLIEKWEL QRSLSKHNRS NIGTEGGPPP FVPFGQKCVS HVQVDSRELD RRKTLQVTMP VKPTNDNDEF EKQRTAAIAE VAKSKETKTF GGGGGGARSN LNMNAAGNRN REVLQKEKST KSEGKHEGVY RELVDEKALK HITEMGFSKE ASRQALMDNG NNLEAALNVL LTSNKQKPVM GPPLRGRGKG RGRIRSEDEE DLGNARPSAP STLFDFLESK MGTLNVEEPK SQPQQLHQGQ YRSSNTEQNG VKDNNHLRHP PRNDTRQPRN EKPPRFQRDS QNSKSVLEGS GLPRNRGSER PSTSSVSEVW AEDRIKCDRP YSRYDRTKDT SYPLGSQHSD GAFKKRDNSM QSRSGKGPSF AEAKENPLPQ GSVDYNNQKR GKRESQTSIP DYFYDRKSQT INNEAFSGIK IEKHFNVNTD YQNPVRSNSF IGVPNGEVEM PLKGRRIGPI KPAGPVTAVP CDDKIFYNSG PKRRSGPIKP EKILESSIPM EYAKMWKPGD ECFALYWEDN KFYRAEVEAL HSSGMTAVVK FIDYGNYEEV LLSNIKPIQT EAWEEEGTYD QTLEFRRGGD GQPRRSTRPT QQFYQPPRAR N
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us. - Characteristics
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- Made in Germany - from design to production - by highly experienced protein experts.
- Human TDRD3 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.
The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).
When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.
The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.
- Purification
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Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
- In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
- Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
- Purity
- >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
- Sterility
- 0.22 μm filtered
- Endotoxin Level
- Protein is endotoxin free.
- Grade
- Crystallography grade
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- Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
- Comment
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In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -80 °C
- Storage Comment
- Store at -80°C.
- Expiry Date
- Unlimited (if stored properly)
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- Target
- TDRD3 (Tudor Domain Containing 3 (TDRD3))
- Alternative Name
- TDRD3 (TDRD3 Products)
- Synonyms
- 4732418C03Rik Protein, 6720468N18 Protein, zgc:77174 Protein, tudor domain containing 3 Protein, tudor domain containing 3 L homeolog Protein, TDRD3 Protein, Tdrd3 Protein, tdrd3 Protein, tdrd3.L Protein
- Background
- Scaffolding protein that specifically recognizes and binds dimethylarginine-containing proteins. In nucleus, acts as a coactivator: recognizes and binds asymmetric dimethylation on the core histone tails associated with transcriptional activation (H3R17me2a and H4R3me2a) and recruits proteins at these arginine-methylated loci. In cytoplasm, may play a role in the assembly and/or disassembly of mRNA stress granules and in the regulation of translation of target mRNAs by binding Arg/Gly-rich motifs (GAR) in dimethylarginine-containing proteins. {ECO:0000269|PubMed:15955813, ECO:0000269|PubMed:18632687, ECO:0000269|PubMed:21172665}.
- Molecular Weight
- 74.1 kDa Including tag.
- UniProt
- Q9H7E2
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