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TMED10 Protein (AA 32-219) (rho-1D4 tag)

Crystallography grade TMED10 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, ELISA, WB, Crys
Catalog No. ABIN3095939
  • Target See all TMED10 Proteins
    TMED10 (Transmembrane Emp24-Like Trafficking Protein 10 (TMED10))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 32-219
    Origin
    • 2
    • 2
    Human
    Source
    • 2
    • 2
    Insect Cells
    Purification tag / Conjugate
    This TMED10 protein is labelled with rho-1D4 tag.
    Application
    SDS-PAGE (SDS), ELISA, Western Blotting (WB), Crystallization (Crys)
    Sequence
    ISFHLPINSR KCLREEIHKD LLVTGAYEIS DQSGGAGGLR SHLKITDSAG HILYSKEDAT KGKFAFTTED YDMFEVCFES KGTGRIPDQL VILDMKHGVE AKNYEEIAKV EKLKPLEVEL RRLEDLSESI VNDFAYMKKR EEEMRDTNES TNTRVLYFSI FSMFCLIGLA TWQVFYLRRF FKAKKLIE
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human TMED10 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Three step purification of membrane proteins expressed in baculovirus infected SF9 insect cells:
    1. Membrane proteins are fractioned by ultracentrifugation and subsequently solubilized with different detergents (detergent screen). Samples are analyzed by Western blot.
    2. The best performing detergent is used for solubilization and the proteins are purified via their rho1D4 tag via two rho1D4 antibody columns: one DTT resistant, the other one not. Eluate fractions are analyzed by Western blot.
    3. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatograph. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin-free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product TMED10 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    TMED10 (Transmembrane Emp24-Like Trafficking Protein 10 (TMED10))
    Alternative Name
    TMED10 (TMED10 Products)
    Synonyms
    tmp21 Protein, P24(DELTA) Protein, S31I125 Protein, S31III125 Protein, TMP21 Protein, Tmp-21-I Protein, p23 Protein, 1110014C03Rik Protein, Tmp21 Protein, p24delta1 Protein, fe06g04 Protein, wu:fb98e10 Protein, wu:fe06g04 Protein, zgc:85681 Protein, transmembrane p24 trafficking protein 10 L homeolog Protein, transmembrane p24 trafficking protein 10 Protein, tmed10.L Protein, TMED10 Protein, Tmed10 Protein, tmed10 Protein
    Background
    Involved in vesicular protein trafficking. Mainly functions in the early secretory pathway. Thought to act as cargo receptor at the lumenal side for incorporation of secretory cargo molecules into transport vesicles and to be involved in vesicle coat formation at the cytoplasmic side. In COPII vesicle-mediated anterograde transport involved in the transport of GPI-anchored proteins and proposed to act together with TMED2 as their cargo receptor, the function specifically implies SEC24C and SEC24D of the COPII vesicle coat and lipid raft-like microdomains of the ER. Recognizes GPI anchors structural remodeled in the ER by PGAP1 and MPPE1 (By similarity). In COPI vesicle-mediated retrograde transport involved in the biogenesis of COPI vesicles and vesicle coat recruitment. On Golgi membranes, acts as primary receptor for ARF1-GDP which is involved in COPI-vesicle formation. Increases coatomer-dependent GTPase-activating activity of ARFGAP2. Involved in trafficking of G protein-coupled receptors (GPCRs). Regulates F2LR1, OPRM1 and P2RY4 exocytic trafficking from the Golgi to the plasma membrane thus contributing to receptor resensitization. Involved in trafficking of amyloid beta A4 protein and soluble APP-beta release (independent of modulation of gamma-secretase activity). As part of the presenilin-dependent gamma-secretase complex regulates gamma-cleavages of the amyloid beta A4 protein to yield amyloid-beta 40 (Abeta40). Involved in organization of the Golgi apparatus. {ECO:0000250, ECO:0000269|PubMed:10052452, ECO:0000269|PubMed:11726511, ECO:0000269|PubMed:16641999, ECO:0000269|PubMed:17288597, ECO:0000269|PubMed:19296914, ECO:0000269|PubMed:20427317, ECO:0000269|PubMed:21219331}.
    Molecular Weight
    22.9 kDa Including tag.
    UniProt
    P49755
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