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TSHZ1 Protein (AA 1-1077) (His tag)

Crystallography grade TSHZ1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3096082
  • Target See all TSHZ1 Proteins
    TSHZ1 (Teashirt Zinc Finger Homeobox 1 (TSHZ1))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-1077
    Origin
    • 1
    • 1
    Human
    Source
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This TSHZ1 protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    MPRRKQQAPR RSAAYVPEEE LKAAEIDEEH VEDDGLSLDI QESEYMCNEE TEIKEAQSYQ NSPVSSATNQ DAGYGSPFSE SSDQLAHFKG SSSREEKEDP QCPDSVSYPQ DSLAQIKAVY ANLFSESCWS SLALDLKKSG STTSTNDASQ KESSAPTPTP PTCPVSTTGP TTSTPSTSCS SSTSHSSTTS TSSSSGYDWH QAALAKTLQQ TSSYGLLPEP SLFSTVQLYR QNNKLYGSVF TGASKFRCKD CSAAYDTLVE LTVHMNETGH YRDDNRDKDS EKTKRWSKPR KRSLMEMEGK EDAQKVLKCM YCGHSFESLQ DLSVHMIKTK HYQKVPLKEP VPAITKLVPS TKKRALQDLA PPCSPEPAGM AAEVALSESA KDQKAANPYV TPNNRYGYQN GASYTWQFEA RKAQILKCME CGSSHDTLQQ LTAHMMVTGH FLKVTTSASK KGKQLVLDPV VEEKIQSIPL PPTTHTRLPA SSIKKQPDSP AGSTTSEEKK EPEKEKPPVA GDAEKIKEES EDSLEKFEPS TLYPYLREED LDDSPKGGLD ILKSLENTVS TAISKAQNGA PSWGGYPSIH AAYQLPGTVK PLPAAVQSVQ VQPSYAGGVK SLSSAEHNAL LHSPGSLTPP PHKSNVSAME ELVEKVTGKV NIKKEERPPE KEKSSLAKAA SPIAKENKDF PKTEEVSGKP QKKGPEAETG KAKKEGPLDV HTPNGTEPLK AKVTNGCNNL GIIMDHSPEP SFINPLSALQ SIMNTHLGKV SKPVSPSLDP LAMLYKISNS MLDKPVYPAT PVKQADAIDR YYYENSDQPI DLTKSKNKPL VSSVADSVAS PLRESALMDI SDMVKNLTGR LTPKSSTPST VSEKSDADGS SFEEALDELS PVHKRKGRQS NWNPQHLLIL QAQFASSLRE TTEGKYIMSD LGPQERVHIS KFTGLSMTTI SHWLANVKYQ LRRTGGTKFL KNLDTGHPVF FCNDCASQFR TASTYISHLE THLGFSLKDL SKLPLNQIQE QQNVSKVLTN KTLGPLGATE EDLGSTFQCK LCNRTFASKH AVKLHLSKTH GKSPEDHLIY VTELEKQ
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human TSHZ1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product TSHZ1 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    TSHZ1 (Teashirt Zinc Finger Homeobox 1 (TSHZ1))
    Alternative Name
    TSHZ1 (TSHZ1 Products)
    Synonyms
    CAA Protein, NY-CO-33 Protein, SDCCAG33 Protein, TSH1 Protein, 5730407I04Rik Protein, D18Bwg1409e Protein, Mtsh1 Protein, Sdccag33 Protein, Tsh1 Protein, mKIAA4206 Protein, teashirt1 Protein, sdccag33 Protein, tsh1 Protein, tshz1a Protein, tsrt Protein, Xtsh1a Protein, tshz1-a Protein, Xtsh1b Protein, tshz1-b Protein, teashirt zinc finger homeobox 1 Protein, teashirt zinc finger family member 1 Protein, teashirt zinc finger homeobox 1 S homeolog Protein, teashirt zinc finger homeobox 1 L homeolog Protein, TSHZ1 Protein, Tshz1 Protein, tshz1 Protein, tshz1.S Protein, tshz1.L Protein
    Background
    Probable transcriptional regulator involved in developmental processes. May act as a transcriptional repressor (Potential). {ECO:0000305}.
    Molecular Weight
    118.9 kDa Including tag.
    UniProt
    Q6ZSZ6
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