HDLBP Protein (AA 2-1268) (His tag)

Catalog No. ABIN3096281

Product Details

Target: HDLBP (High Density Lipoprotein Binding Protein (HDLBP))

Protein Type: Recombinant

Protein Characteristics: AA 2-1268

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This HDLBP protein is labelled with His tag.

Application: SDS-PAGE (SDS), Western Blotting (WB), Crystallization (Crys), ELISA

Sequence: SSVAVLTQES FAEHRSGLVP QQIKVATLNS EEESDPPTYK DAFPPLPEKA ACLESAQEPS GAWGNKIRPI KASVITQVFH VPLEERKYKD MNQFGEGEQA KICLEIMQRT GAHLELSLAK DQGLSIMVSG KLDAVMKARK DIVARLQTQA SATVAIPKEH HRFVIGKNGE KLQDLELKTA TKIQIPRPDD PSNQIKITGT KEGIEKARHE VLLISAEQDK RAVERLEVEK AFHPFIAGPY NRLVGEIMQE TGTRINIPPP SVNRTEIVFT GEKEQLAQAV ARIKKIYEEK KKKTTTIAVE VKKSQHKYVI GPKGNSLQEI LERTGVSVEI PPSDSISETV ILRGEPEKLG QALTEVYAKA NSFTVSSVAA PSWLHRFIIG KKGQNLAKIT QQMPKVHIEF TEGEDKITLE GPTEDVNVAQ EQIEGMVKDL INRMDYVEIN IDHKFHRHLI GKSGANINRI KDQYKVSVRI PPDSEKSNLI RIEGDPQGVQ QAKRELLELA SRMENERTKD LIIEQRFHRT IIGQKGERIR EIRDKFPEVI INFPDPAQKS DIVQLRGPKN EVEKCTKYMQ KMVADLVENS YSISVPIFKQ FHKNIIGKGG ANIKKIREES NTKIDLPAEN SNSETIIITG KRANCEAARS RILSIQKDLA NIAEVEVSIP AKLHNSLIGT KGRLIRSIME ECGGVHIHFP VEGSGSDTVV IRGPSSDVEK AKKQLLHLAE EKQTKSFTVD IRAKPEYHKF LIGKGGGKIR KVRDSTGARV IFPAAEDKDQ DLITIIGKED AVREAQKELE ALIQNLDNVV EDSMLVDPKH HRHFVIRRGQ VLREIAEEYG GVMVSFPRSG TQSDKVTLKG AKDCVEAAKK RIQEIIEDLE AQVTLECAIP QKFHRSVMGP KGSRIQQITR DFSVQIKFPD REENAVHSTE PVVQENGDEA GEGREAKDCD PGSPRRCDII IISGRKEKCE AAKEALEALV PVTIEVEVPF DLHRYVIGQK GSGIRKMMDE FEVNIHVPAP ELQSDIIAIT GLAANLDRAK AGLLERVKEL QAEQEDRALR SFKLSVTVDP KYHPKIIGRK GAVITQIRLE HDVNIQFPDK DDGNQPQDQI TITGYEKNTE AARDAILRIV GELEQMVSED VPLDHRVHAR IIGARGKAIR KIMDEFKVDI RFPQSGAPDP NCVTVTGLPE NVEEAIDHIL NLEEEYLADV VDSEALQVYM KPPAHEEAKA PSRGFVVRDA PWTASSSEKA PDMSSSEEFP SFGAQVAPKT LPWGPKR
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human HDLBP Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: HDLBP (High Density Lipoprotein Binding Protein (HDLBP))

Alternative Name: HDLBP (HDLBP Products)

Synonyms: HBP Protein, PRO2900 Protein, VGL Protein, 1110005P14Rik Protein, AA960365 Protein, AI118566 Protein, D1Ertd101e Protein, HDLBP Protein, vigilin Protein, cb591 Protein, cb811 Protein, hdlbp Protein, wu:fb36d01 Protein, wu:fb94h08 Protein, wu:fc23d06 Protein, wu:fc75h07 Protein, high density lipoprotein binding protein Protein, high density lipoprotein (HDL) binding protein Protein, vigilin Protein, high density lipoprotein binding protein (vigilin) S homeolog Protein, high density lipoprotein binding protein a Protein, HDLBP Protein, Hdlbp Protein, hdlbp.S Protein, hdlbpa Protein

Background: Appears to play a role in cell sterol metabolism. It may function to protect cells from over-accumulation of cholesterol.

Molecular Weight: 142.3 kDa Including tag.

UniProt: Q00341