Villin 1 Protein (VIL1) (AA 2-827) (His tag)

Catalog No. ABIN3096362

Product Details

Target: Villin 1 (VIL1)

Protein Type: Recombinant

Protein Characteristics: AA 2-827

Origin: Human

Source: Insect Cells

Purification tag / Conjugate: This Villin 1 protein is labelled with His tag.

Application: Western Blotting (WB), ELISA, SDS-PAGE (SDS), Crystallization (Crys)

Sequence: TKLSAQVKGS LNITTPGLQI WRIEAMQMVP VPSSTFGSFF DGDCYIILAI HKTASSLSYD IHYWIGQDSS LDEQGAAAIY TTQMDDFLKG RAVQHREVQG NESEAFRGYF KQGLVIRKGG VASGMKHVET NSYDVQRLLH VKGKRNVVAG EVEMSWKSFN RGDVFLLDLG KLIIQWNGPE STRMERLRGM TLAKEIRDQE RGGRTYVGVV DGENELASPK LMEVMNHVLG KRRELKAAVP DTVVEPALKA ALKLYHVSDS EGNLVVREVA TRPLTQDLLS HEDCYILDQG GLKIYVWKGK KANEQEKKGA MSHALNFIKA KQYPPSTQVE VQNDGAESAV FQQLFQKWTA SNRTSGLGKT HTVGSVAKVE QVKFDATSMH VKPQVAAQQK MVDDGSGEVQ VWRIENLELV PVDSKWLGHF YGGDCYLLLY TYLIGEKQHY LLYVWQGSQA SQDEITASAY QAVILDQKYN GEPVQIRVPM GKEPPHLMSI FKGRMVVYQG GTSRTNNLET GPSTRLFQVQ GTGANNTKAF EVPARANFLN SNDVFVLKTQ SCCYLWCGKG CSGDEREMAK MVADTISRTE KQVVVEGQEP ANFWMALGGK APYANTKRLQ EENLVITPRL FECSNKTGRF LATEIPDFNQ DDLEEDDVFL LDVWDQVFFW IGKHANEEEK KAAATTAQEY LKTHPSGRDP ETPIIVVKQG HEPPTFTGWF LAWDPFKWSN TKSYEDLKAE LGNSRDWSQI TAEVTSPKVD VFNANSNLSS GPLPIFPLEQ LVNKPVEELP EGVDPSRKEE HLSIEDFTQA FGMTPAAFSA LPRWKQQNLK KEKGLF
Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.

Characteristics:

• Made in Germany - from design to production - by highly experienced protein experts.
• Human VIL1 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in baculovirus infected SF9 insect cells:

1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Sterility: 0.22 μm filtered

Endotoxin Level: Protein is endotoxin free.

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.

Comment:

In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: Villin 1 (VIL1)

Alternative Name: VIL1 (VIL1 Products)

Synonyms: D2S1471 Protein, VIL Protein, Vil Protein, vil1 Protein, MGC64339 Protein, VIL1 Protein, vil1l Protein, zgc:55779 Protein, villin 1 Protein, villin 1 S homeolog Protein, VIL1 Protein, Vil1 Protein, vil1.S Protein, vil1 Protein

Background: Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA), binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Upon S.flexneri cell infection, its actin-severing activity enhances actin-based motility of the bacteria and plays a role during the dissemination. {ECO:0000269|PubMed:11500485, ECO:0000269|PubMed:14594952, ECO:0000269|PubMed:15084600, ECO:0000269|PubMed:15272027, ECO:0000269|PubMed:15342783, ECO:0000269|PubMed:16921170, ECO:0000269|PubMed:17182858, ECO:0000269|PubMed:17229814, ECO:0000269|PubMed:17606613, ECO:0000269|PubMed:18054784, ECO:0000269|PubMed:18198174, ECO:0000269|PubMed:19808673, ECO:0000269|PubMed:3087992}.

Molecular Weight: 93.5 kDa Including tag.

UniProt: P09327

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization