YEATS2 Protein (AA 1-1422) (Strep Tag)

Catalog No. ABIN3096419

Product Details

Target: YEATS2 (YEATS Domain Containing 2 (YEATS2))

Protein Type: Recombinant

Protein Characteristics: AA 1-1422

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This YEATS2 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MSGIKRTIKE TDPDYEDVSV ALPNKRHKAI ENSARDAAVQ KIETIIKEQF ALEMKNKEHE IEVIDQRLIE ARRMMDKLRA CIVANYYASA GLLKVSEGSK TCDTMVFNHP AIKKFLESPS RSSSPANQRA ETPSANHSES DSLSQHNDFL SDKDNNSNMD IEERLSNNME QRPSRNTGRD TSRITGSHKT EQRNADLTDE TSRLFVKKTI VVGNVSKYIP PDKREENDQS THKWMVYVRG SRREPSINHF VKKVWFFLHP SYKPNDLVEV REPPFHLTRR GWGEFPVRVQ VHFKDSQNKR IDIIHNLKLD RTYTGLQTLG AETVVDVELH RHSLGEDCIY PQSSESDISD APPSLPLTIP APVKASSPIK QSHEPVPDTS VEKGFPASTE AERHTPFYAL PSSLERTPTK MTTSQKVTFC SHGNSAFQPI ASSCKIVPQS QVPNPESPGK SFQPITMSCK IVSGSPISTP SPSPLPRTPT STPVHVKQGT AGSVINNPYV IMDKQPGQVI GATTPSTGSP TNKISTASQV SQGTGSPVPK IHGSSFVTST VKQEDSLFAS MPPLCPIGSH PKVQSPKPIT GGLGAFTKVI IKQEPGEAPH VPATGAASQS PLPQYVTVKG GHMIAVSPQK QVITPGEGIA QSAKVQPSKV VGVPVGSALP STVKQAVAIS GGQILVAKAS SSVSKAVGPK QVVTQGVAKA IVSGGGGTIV AQPVQTLTKA QVTAAGPQKS GSQGSVMATL QLPATNLANL ANLPPGTKLY LTTNSKNPSG KGKLLLIPQG AILRATNNAN LQSGSAASGG SGAGGGGGGG GGGGSGSGGG GSTGGGGGTA GGGTQSTAGP GGISQHLTYT SYILKQTPQG TFLVGQPSPQ TSGKQLTTGS VVQGTLGVST SSAQGQQTLK VISGQKTTLF TQAAHGGQAS LMKISDSTLK TVPATSQLSK PGTTMLRVAG GVITTATSPA VALSANGPAQ QSEGMAPVSS STVSSVTKTS GQQQVCVSQA TVGTCKAATP TVVSATSLVP TPNPISGKAT VSGLLKIHSS QSSPQQAVLT IPSQLKPLSV NTSGGVQTIL MPVNKVVQSF STSKPPAILP VAAPTPVVPS SAPAAVAKVK TEPETPGPSC LSQEGQTAVK TEESSELGNY VIKIDHLETI QQLLTAVVKK IPLITAKSED ASCFSAKSVE QYYGWNIGKR RAAEWQRAMT MRKVLQEILE KNPRFHHLTP LKTKHIAHWC RCHGYTPPDP ESLRNDGDSI EDVLTQIDSE PECPSSFSSA DNLCRKLEDL QQFQKREPEN EEEVDILSLS EPVKINIKKE QEEKQEEVKF YLPPTPGSEF IGDVTQKIGI TLQPVALHRN VYASVVEDMI LKATEQLVND ILRQALAVGY QTASHNRIPK EITVSNIHQA ICNIPFLDFL TNKHMGILNE DQ
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: YEATS2 (YEATS Domain Containing 2 (YEATS2))

Alternative Name: YEATS2 (YEATS2 Products)

Synonyms: MGC68945 Protein, RGD1566176 Protein, BC042768 Protein, mKIAA1197 Protein, YEATS domain containing 2 L homeolog Protein, YEATS domain containing 2 Protein, yeats2.L Protein, YEATS2 Protein, Yeats2 Protein, yeats2 Protein

Background: YEATS domain-containing protein 2,FUNCTION: Chromatin reader component of the ATAC complex, a complex with histone acetyltransferase activity on histones H3 and H4 (PubMed:18838386, PubMed:19103755, PubMed:27103431). YEATS2 specifically recognizes and binds histone H3 crotonylated at 'Lys-27' (H3K27cr) (PubMed:27103431). Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors (PubMed:27103431). {ECO:0000269|PubMed:18838386, ECO:0000269|PubMed:19103755, ECO:0000269|PubMed:27103431}.

Molecular Weight: 150.8 kDa

UniProt: Q9ULM3