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ZFP91 Protein (AA 1-1191) (His tag)

Crystallography grade ZFP91 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Catalog No. ABIN3096492
  • Target See all ZFP91 Proteins
    ZFP91 (ZFP91 Zinc Finger Protein (ZFP91))
    Protein Type
    Recombinant
    Protein Characteristics
    AA 1-1191
    Origin
    Human
    Source
    • 1
    • 1
    • 1
    Insect Cells
    Purification tag / Conjugate
    This ZFP91 protein is labelled with His tag.
    Application
    ELISA, Western Blotting (WB), Crystallization (Crys), SDS-PAGE (SDS)
    Sequence
    MPGTPGSLEM GLLTFRDVAI EFSPEEWQCL DTAQQNLYRN VMLENYRNLA FLGIALSKPD LITYLEQGKE PWNMKQHEMV DEPTGICPHF PQDFWPEQSM EDSFQKVLLR KYEKCGHENL QLRKGCKSVD ECKVHKEGYN KLNQCLTTAQ SKVFQCGKYL KVFYKFLNSN RHTIRHTGKK CFKCKKCVKS FCIRLHKTQH KCVYITEKSC KCKECEKTFH WSSTLTNHKE IHTEDKPYKC EECGKAFKQL STLTTHKIIC AKEKIYKCEE CGKAFLWSST LTRHKRIHTG EKPYKCEECG KAFSHSSTLA KHKRIHTGEK PYKCEECGKA FSRSSTLAKH KRIHTGEKPY KCKECGKAFS NSSTLANHKI THTEEKPYKC KECDKAFKRL STLTKHKIIH AGEKLYKCEE CGKAFNRSSN LTIHKFIHTG EKPYKCEECG KAFNWSSSLT KHKRFHTREK PFKCKECGKA FIWSSTLTRH KRIHTGEKPY KCEECGKAFR QSSTLTKHKI IHTGEKPYKF EECGKAFRQS LTLNKHKIIH SREKPYKCKE CGKAFKQFST LTTHKIIHAG KKLYKCEECG KAFNHSSSLS THKIIHTGEK SYKCEECGKA FLWSSTLRRH KRIHTGEKPY KCEECGKAFS HSSALAKHKR IHTGEKPYKC KECGKAFSNS STLANHKITH TEEKPYKCKE CDKTFKRLST LTKHKIIHAG EKLYKCEECG KAFNRSSNLT IHKFIHTGEK PYKCEECGKA FNWSSSLTKH KRIHTREKPF KCKECGKAFI WSSTLTRHKR IHTGEKPYKC EECGKAFSRS STLTKHKTIH TGEKPYKCKE CGKAFKHSSA LAKHKIIHAG EKLYKCEECG KAFNQSSNLT THKIIHTKEK PSKSEECDKA FIWSSTLTEH KRIHTREKTY KCEECGKAFS QPSHLTTHKR MHTGEKPYKC EECGKAFSQS STLTTHKIIH TGEKPYKCEE CGKAFRKSST LTEHKIIHTG EKPYKCEECG KAFSQSSTLT RHTRMHTGEK PYKCEECGKA FNRSSKLTTH KIIHTGEKPY KCEECGKAFI SSSTLNGHKR IHTREKPYKC EECGKAFSQS STLTRHKRLH TGEKPYKCGE CGKAFKESSA LTKHKIIHTG EKPYKCEKCG KAFNQSSILT NHKKIHTITP VIPLLWEAEA GGSRGQEMET ILANTVKPLL Y
    Sequence without tag. Tag location is at the discretion of the manufacturer. If you have a special request, please contact us.
    Characteristics
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Human ZNF91 Protein (raised in Insect Cells) purified by multi-step, protein-specific process to ensure crystallization grade.
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a made to order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

    The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

    In the unlikely event that the protein cannot be expressed or purified we do not charge anything (other companies might charge you for any performed steps in the expression process for custom-made proteins, e.g. fees might apply for the expression plasmid, the first expression experiments or purification optimization).

    When you order this made-to-order protein you will only pay upon receival of the correctly folded protein. With no financial risk on your end you can rest assured that our experienced protein experts will do everything to make sure that you receive the protein you ordered.

    The concentration of our recombinant proteins is measured using the absorbance at 280nm. The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.

    The concentration of the protein is calculated using its specific absorption coefficient. We use the Expasy's protparam tool to determine the absorption coefficient of each protein.

    Purification
    Two step purification of proteins expressed in baculovirus infected SF9 insect cells:
    1. In a first purification step, the protein is purified from the cleared cell lysate using three different His-tag capture materials: high yield, EDTA resistant, or DTT resistant. Eluate fractions are analyzed by SDS-PAGE.
    2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.
    Purity
    >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
    Sterility
    0.22 μm filtered
    Endotoxin Level
    Protein is endotoxin free.
    Grade
    Crystallography grade
    Top Product
    Discover our top product ZFP91 Protein
  • Application Notes
    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a gurantee though.
    Comment

    In cases in which it is highly likely that the recombinant protein with the default tag will be insoluble our protein lab may suggest a higher molecular weight tag (e.g. GST-tag) instead to increase solubility. We will discuss all possible options with you in detail to assure that you receive your protein of interest.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    100 mM NaCL, 20 mM Hepes, 10% glycerol. pH value is at the discretion of the manufacturer.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -80 °C
    Storage Comment
    Store at -80°C.
    Expiry Date
    Unlimited (if stored properly)
  • Target
    ZFP91 (ZFP91 Zinc Finger Protein (ZFP91))
    Alternative Name
    ZNF91 (ZFP91 Products)
    Synonyms
    CNTF Protein, DMS-8 Protein, DSM-8 Protein, PZF Protein, ZFP-91 Protein, ZNF757 Protein, HPF7 Protein, HTF10 Protein, 9130014I08Rik Protein, A530054C17Rik Protein, AL024263 Protein, AW545902 Protein, Pzf Protein, Zfp-91 Protein, ZFP91 zinc finger protein Protein, zinc finger protein 91 Protein, ZFP91 Protein, ZNF91 Protein, Zfp91 Protein
    Background
    Transcription factor specifically required to repress SINE-VNTR-Alu (SVA) retrotransposons: recognizes and binds SVA sequences and represses their expression by recruiting a repressive complex containing TRIM28/KAP1 (PubMed:25274305). May also bind the promoter of the FCGR2B gene, leading to repress its expression, however, additional evidences are required to confirm this result in vivo (PubMed:11470777). {ECO:0000269|PubMed:25274305, ECO:0000305|PubMed:11470777}.
    Molecular Weight
    138.2 kDa Including tag.
    UniProt
    Q05481
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