ZMYM6 Protein (AA 1-1325) (Strep Tag)

Catalog No. ABIN3096521

Product Details

Target: ZMYM6 (Zinc Finger, MYM-Type 6 (ZMYM6))

Protein Type: Recombinant

Protein Characteristics: AA 1-1325

Origin: Human

Source: Tobacco (Nicotiana tabacum)

Purification tag / Conjugate: This ZMYM6 protein is labelled with Strep Tag.

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS)

Sequence: MKEPLDGECG KAVVPQQELL DKIKEEPDNA QEYGCVQQPK TQESKLKIGG VSSVNERPIA QQLNPGFQLS FASSGPSVLL PSVPAVAIKV FCSGCKKMLY KGQTAYHKTG STQLFCSTRC ITRHSSPACL PPPPKKTCTN CSKDILNPKD VITTRFENSY PSKDFCSQSC LSSYELKKKP VVTIYTKSIS TKCSMCQKNA DTRFEVKYQN VVHGLCSDAC FSKFHSTNNL TMNCCENCGS YCYSSSGPCQ SQKVFSSTSV TAYKQNSAQI PPYALGKSLR PSAEMIETTN DSGKTELFCS INCLSAYRVK TVTSSGVQVS CHSCKTSAIP QYHLAMSNGT IYSFCSSSCV VAFQNVFSKP KGTNSSAVPL SQGQVVVSPP SSRSAVSIGG GNTSAVSPSS IRGSAAASLQ PLAEQSQQVA LTHTVVKLKC QHCNHLFATK PELLFYKGKM FLFCGKNCSD EYKKKNKVVA MCDYCKLQKI IKETVRFSGV DKPFCSEVCK FLSARDFGER WGNYCKMCSY CSQTSPNLVE NRLEGKLEEF CCEDCMSKFT VLFYQMAKCD GCKRQGKLSE SIKWRGNIKH FCNLFCVLEF CHQQIMNDCL PQNKVNISKA KTAVTELPSA RTDTTPVITS VMSLAKIPAT LSTGNTNSVL KGAVTKEAAK IIQDESTQED AMKFPSSQSS QPSRLLKNKG ISCKPVTQTK ATSCKPHTQH KECQTDLPMP NEKNDAELDS PPSKKKRLGF FQTYDTEYLK VGFIICPGSK ESSPRPQCVI CGEILSSENM KPANLSHHLK TKHSELENKP VDFFEQKSLE MECQNSSLKK CLLVEKSLVK ASYLIAFQTA ASKKPFSIAE ELIKPYLVEM CSEVLGSSAG DKMKTIPLSN VTIQHRIDEL SADIEDQLIQ KVRESKWFAL QIDESSEISN ITLLLCYIRF IDYDCRDVKE ELLFCIEMPT QITGFEIFEL INKYIDSKSL NWKHCVGLCT DGAASMTGRY SGLKAKIQEV AMNTAAFTHC FIHRERLVAE KLSPCLHKIL LQSAQILSFI KSNALNSRML TILCEEMGSE HVSLPLHAEV RWISRGRMLK RLFELRHEIE IFLSQKHSDL AKYFHDEEWV GKLAYLSDIF SLINELNLSL QGTLTTFFNL CNKIDVFKRK LKMWLKRTQE NDYDMFPSFS EFSNSSGLNM TDITRIIFEH LEGLSQVFSD CFPPEQDLRS GNLWIIHPFM NHQNNNLTDF EEEKLTELSS DLGLQALFKS VSVTQFWINA KTSYPELHER AMKFLLPFST VYLCDAAFSA LTESKQKNLL GSGPALRLAV TSLIPRIEKL VKEKE
Sequence without tag. The proposed Strep-Tag is based on experience s with the expression system, a different complexity of the protein could make another tag necessary. In case you have a special request, please contact us.

Characteristics: Key Benefits:

• Made in Germany - from design to production - by highly experienced protein experts.
• Protein expressed with ALiCE® and purified by multi-step, protein-specific process to ensure correct folding and modification.
• These proteins are normally active (enzymatically functional) as our customers have reported (not tested by us and not guaranteed).
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a made-to-order protein and will be made for the first time for your order. Our experts in the lab will ensure that you receive a correctly folded protein.

The big advantage of ordering our made-to-order proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Expression System:

• ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
• During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Concentration:

• The concentration of our recombinant proteins is measured using the absorbance at 280nm.
• The protein's absorbance will be measured in several dilutions and is measured against its specific reference buffer.
• We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

Purification: Two step purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®):

1. In a first purification step, the protein is purified from the cleared cell lysate using StrepTag capture material. Eluate fractions are analyzed by SDS-PAGE.
2. Protein containing fractions of the best purification are subjected to second purification step through size exclusion chromatography. Eluate fractions are analyzed by SDS-PAGE and Western blot.

Purity: >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.

Endotoxin Level: Low Endotoxin less than 1 EU/mg (< 0.1 ng/mg)

Grade: Crystallography grade

Application Details

Application Notes: In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Comment:

ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer. If you have a special request, please contact us.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: Unlimited (if stored properly)

Target Details

Target: ZMYM6 (Zinc Finger, MYM-Type 6 (ZMYM6))

Alternative Name: ZMYM6 (ZMYM6 Products)

Synonyms: MYM Protein, ZNF258 Protein, Buster2 Protein, ZNF198L4 Protein, Zfp258 Protein, AI593204 Protein, AI661340 Protein, D4Wsu24e Protein, 9330177P20Rik Protein, RGD1561833 Protein, DKFZp468A089 Protein, zinc finger MYM-type containing 6 Protein, zinc finger, MYM-type 6 Protein, ZMYM6 Protein, Zmym6 Protein

Background: Zinc finger MYM-type protein 6 (Transposon-derived Buster2 transposase-like protein) (Zinc finger protein 258),FUNCTION: Plays a role in the regulation of cell morphology and cytoskeletal organization. {ECO:0000269|PubMed:21834987}.

Molecular Weight: 148.1 kDa

UniProt: O95789